ProtParam
User-provided sequence:
10 20 30 40 50 60
MPVKVLMPAL SPTMKSGTIA KWHKNAGDAV KPGDIVADIE TDKAVIEFEY ADEPGVMYKI
70 80 90 100 110 120
LKEEGSKNVA VNQSIAVIKV DGDEEAALLE MVHSAEGASG SVSNEAASAA LQATPAKVAG
130 140 150 160 170 180
DMVAPSSANK ASETAHITSG SDRVKASPLA KKLAAQLSVD ISKITGSGPY GRVVKADVLG
190 200 210 220 230 240
ASVPTSDTTI QEGSRVVEVS TMRKVISERL AESKRNIPHF YLAIDCMVGE LLEVRSRINS
250 260 270 280 290 300
NAEALGTKIT VNDLVIKATA LAAREFPEVN ALWAGDKIVY HQNVDIAFAV ALDDGLLTPV
310 320 330 340 350 360
IAGADKMTLS ELSKTAKSLV ARAKDRKLLP HEFQGGCLTI SNLGMFCIKE FYAIINPPQS
370 380 390 400 410 420
CIMAVGQSEK RPVVVDNCVV AADVMSVTLS VDHRVIDGAL AAKFLNRFKF YIENPLAMLA
References and
documentation are available.
Number of amino acids: 420
Molecular weight: 44574.43
Theoretical pI: 5.90
Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 45
Atomic composition:
Carbon C 1966
Hydrogen H 3213
Nitrogen N 533
Oxygen O 605
Sulfur S 18
Formula: C
1966H
3213N
533O
605S
18
Total number of atoms: 6335
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 21680
Abs 0.1% (=1 g/l) 0.486, assuming all pairs of Cys residues form cystines
Ext. coefficient 21430
Abs 0.1% (=1 g/l) 0.481, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.72
This classifies the protein as stable.
Aliphatic index: 99.67
Grand average of hydropathicity (GRAVY): 0.105