ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPVLGQIWEL SDSQKVGSLD KRGAFVAFKL VAAAQQGKPV ANSSLYDSSL QPPRFAPPVP 

        70         80         90        100        110        120 
PANMQHHFQP SFPSVGRAPP VPPPPHPSHP YSISSQQSSP AHPSSAFPPQ WPITPTDQAK 

       130        140        150        160        170        180 
YDSIFQSLNP VNGKLSGAHV RPVLMNSGLD AHALARIWEL SDQDKDGNLD RIEMSVALHL 

       190        200        210        220        230        240 
VYRSLQSDPV PAQLPPNLIH PSKAMYAHSS PNFAAPPHPP RPMMGSRAGS VTSLDDVNMS 

       250        260        270        280        290        300 
QSYSATMPRA YPPQPGRAYS AQAHVNGSRT SGASTPISAS HSIHSFPAGE WPINTGDYAD 

       310        320        330        340        350        360 
QFAQTDTNKD GLVDGMDMRA PMMTTGLSAQ ILAHVWALAD IKKCGQLNLE QFALTMHLLD 

       370        380        390        400        410        420 
MAKRGESIPS ELPLHLIPPS FRPPTEPSAL HHPAQSVSTP QLPEATSMEI KEALEGENEE 

       430        440        450        460        470        480 
MKQLAESIQS MLVERKTAEE AVLQLEADMT IKNSSIKNLQ VELATLESTV KQLERQKGEA 

       490        500        510        520        530        540 
TRRLADYDTQ IEQLESACKA QKETKEDTEK RMQQIDEDAK NAEDCKANDE KEMEELKKEI 

       550        560        570        580        590        600 
EMLDNQFKTV RGEIVKETSQ REQKVAELTT LERKEARDQI QMEKLDAAIE NTTKLTEQVS 

       610        620        630        640        650        660 
DAVEKSEEEM VQILRSQQRL LSTVIDQSLL SDDTVYGETA GTSSQNHVQQ PPDPFASARA 

       670        680        690        700        710        720 
NPAADPFAQV DQFGSSGHFD AAFPTDPFAQ GGFPSDSGFA QSAPAKPAPP RPAPPKSARE 

       730        740        750 
TPVNDPFAPS QGQSTQPAGF ADFADFGSAF N 

References and documentation are available.

Number of amino acids: 751

Molecular weight: 81849.46

Theoretical pI: 5.13

Amino acid composition: 
Ala (A)  82	 10.9%
Arg (R)  29	  3.9%
Asn (N)  24	  3.2%
Asp (D)  45	  6.0%
Cys (C)   3	  0.4%
Gln (Q)  56	  7.5%
Glu (E)  52	  6.9%
Gly (G)  34	  4.5%
His (H)  21	  2.8%
Ile (I)  26	  3.5%
Leu (L)  55	  7.3%
Lys (K)  37	  4.9%
Met (M)  24	  3.2%
Phe (F)  26	  3.5%
Pro (P)  74	  9.9%
Ser (S)  75	 10.0%
Thr (T)  37	  4.9%
Trp (W)   5	  0.7%
Tyr (Y)  11	  1.5%
Val (V)  35	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 97
Total number of positively charged residues (Arg + Lys): 66

Atomic composition:

Carbon      C	      3573
Hydrogen    H	      5606
Nitrogen    N	      1002
Oxygen      O	      1149
Sulfur      S	        27

Formula: C3573H5606N1002O1149S27
Total number of atoms: 11357

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    44015
Abs 0.1% (=1 g/l)   0.538, assuming all pairs of Cys residues form cystines


Ext. coefficient    43890
Abs 0.1% (=1 g/l)   0.536, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 55.07
This classifies the protein as unstable.



Aliphatic index: 66.50

Grand average of hydropathicity (GRAVY): -0.601