ProtParam
User-provided sequence:
10 20 30 40 50 60
MPVVHVIDVE SGNLQSLTNA IEHLGYEVQL VKSPKDFNIS GTSRLILPGV GNYGHFVDNL
70 80 90 100 110 120
FNRGFEKPIR EYIESGKPIM GICVGLQALF AGSVESPKST GLNYIDFKLS RFDDSEKPVP
130 140 150 160 170 180
EIGWNSCIPS ENLFFGLDPY KRYYFVHSFA AILNSEKKKN LENDGWKIAK AKYGSEEFIA
190 200 210 220 230 240
AVNKNNIFAT QFHPEKSGKA GLNVIENFLK QQSPPIPNYS AEEKELLMND YSNYGLTRRI
250 260 270 280 290 300
IACLDVRTND QGDLVVTKGD QYDVREKSDG KGVRNLGKPV QLAQKYYQQG ADEVTFLNIT
310 320 330 340 350 360
SFRDCPLKDT PMLEVLKQAA KTVFVPLTVG GGIKDIVDVD GTKIPALEVA SLYFRSGADK
370 380 390 400 410 420
VSIGTDAVYA AEKYYELGNR GDGTSPIETI SKAYGAQAVV ISVDPKRVYV NSQADTKNKV
430 440 450 460 470 480
FETEYPGPNG EKYCWYQCTI KGGRESRDLG VWELTRACEA LGAGEILLNC IDKDGSNSGY
490 500 510 520 530 540
DLELIEHVKD AVKIPVIASS GAGVPEHFEE AFLKTRADAC LGAGMFHRGE FTVNDVKEYL
550
LEHGLKVRMD EE
References and
documentation are available.
Number of amino acids: 552
Molecular weight: 61068.11
Theoretical pI: 5.33
Total number of negatively charged residues (Asp + Glu): 77
Total number of positively charged residues (Arg + Lys): 63
Atomic composition:
Carbon C 2733
Hydrogen H 4257
Nitrogen N 723
Oxygen O 834
Sulfur S 15
Formula: C
2733H
4257N
723O
834S
15
Total number of atoms: 8562
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 59750
Abs 0.1% (=1 g/l) 0.978, assuming all pairs of Cys residues form cystines
Ext. coefficient 59250
Abs 0.1% (=1 g/l) 0.970, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.61
This classifies the protein as stable.
Aliphatic index: 84.22
Grand average of hydropathicity (GRAVY): -0.340