ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPYSHGKKLK PSLKLAKTIS TSSFVSSTTS NSFSPLEDST SASSSTSSSS SGKSVRFAAH 

        70         80         90        100        110        120 
LYTVKKFNTK LAPISISEKA ASNLTRNLHN NAIPLTFPFI GGEDHRYSLD ILDYSDLEYD 

       130        140        150        160        170        180 
NKDVEYDNES DVEDNAMLMH DRSMFIEKEI LCFGEEETFD MADWKLVSNN LNPFKSDYKV 

       190        200        210        220        230        240 
DVTGLEDKIF KYLNGQNIKV HSLELSDPVS YEDICSNNFG NCQIWGLIFV NNLNFEKKIE 

       250        260        270        280        290        300 
IKFTLNNWAD IHYINAHYNK SVTPHVDEFK FIIDISALKL NLISKNLIYT NFFERKTTCL 

       310        320        330        340        350        360 
LNLQFCCRYD VNGFEYRSFY DNNDYKNYEI TISLSAINLN RAVSNSSIFN SNLGPSKMGA 

       370        380        390        400        410        420 
SNAEVTMSKN NENSKKPLRK FIKDTDYYND SPLKHKFYQS FETKAACKTE PVSQTFKAET 

       430        440        450        460        470        480 
IDCEIEPFNY FFEPPDSQTN EDMSDSSYDL SLQDFNYWEF SNHGLGKALA DSDILQFKNY 

       490        500        510        520        530        540 
PKPEPFSRPP IIDDTFTLNT DDRTLGSKTQ KLEDNLAKEW KSAKTRTTLN ETPLHDDEHR 

       550        560        570        580        590        600 
TSFTYTTWNN STDTLMKRKE ERPVESASCS QLSIATIKAE EDLLYQDYIN SGRESSSPEI 

       610        620        630        640 
SPLNTTTSLP FFPGDNMSDS SGEYEERTSL SPNKIHIFRD YFYKSPSP 

References and documentation are available.

Number of amino acids: 648

Molecular weight: 74146.30

Theoretical pI: 5.09

Amino acid composition: 
Ala (A)  27	  4.2%
Arg (R)  18	  2.8%
Asn (N)  56	  8.6%
Asp (D)  48	  7.4%
Cys (C)   9	  1.4%
Gln (Q)  11	  1.7%
Glu (E)  46	  7.1%
Gly (G)  18	  2.8%
His (H)  14	  2.2%
Ile (I)  38	  5.9%
Leu (L)  53	  8.2%
Lys (K)  51	  7.9%
Met (M)  10	  1.5%
Phe (F)  40	  6.2%
Pro (P)  30	  4.6%
Ser (S)  80	 12.3%
Thr (T)  45	  6.9%
Trp (W)   6	  0.9%
Tyr (Y)  30	  4.6%
Val (V)  18	  2.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 94
Total number of positively charged residues (Arg + Lys): 69

Atomic composition:

Carbon      C	      3295
Hydrogen    H	      5016
Nitrogen    N	       854
Oxygen      O	      1059
Sulfur      S	        19

Formula: C3295H5016N854O1059S19
Total number of atoms: 10243

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    78200
Abs 0.1% (=1 g/l)   1.055, assuming all pairs of Cys residues form cystines


Ext. coefficient    77700
Abs 0.1% (=1 g/l)   1.048, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.15
This classifies the protein as unstable.



Aliphatic index: 66.99

Grand average of hydropathicity (GRAVY): -0.669