ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDAKP INLGLWDTAG 

        70         80         90        100        110        120 
QEDYDRLRPL SYPQTDVFLI CFSLVNPASF ENVRAKWFPE VRHHCPSVPI ILVGTKLDLR 

       130        140        150        160        170        180 
DDKQTIEKLK DKKLTPITYP QGLAMAKEIA AVKYLECSAL TQKGLKTVFD EAIRSVLCPV 

       190 
VRGPKRHKCA LL 

References and documentation are available.

Number of amino acids: 192

Molecular weight: 21359.00

Theoretical pI: 8.63

Amino acid composition: 
Ala (A)  15	  7.8%
Arg (R)   8	  4.2%
Asn (N)   6	  3.1%
Asp (D)  12	  6.2%
Cys (C)   7	  3.6%
Gln (Q)   6	  3.1%
Glu (E)   8	  4.2%
Gly (G)  10	  5.2%
His (H)   3	  1.6%
Ile (I)  11	  5.7%
Leu (L)  20	 10.4%
Lys (K)  16	  8.3%
Met (M)   3	  1.6%
Phe (F)   7	  3.6%
Pro (P)  13	  6.8%
Ser (S)   8	  4.2%
Thr (T)  12	  6.2%
Trp (W)   2	  1.0%
Tyr (Y)   7	  3.6%
Val (V)  18	  9.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 20
Total number of positively charged residues (Arg + Lys): 24

Atomic composition:

Carbon      C	       966
Hydrogen    H	      1542
Nitrogen    N	       252
Oxygen      O	       272
Sulfur      S	        10

Formula: C966H1542N252O272S10
Total number of atoms: 3042

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    21805
Abs 0.1% (=1 g/l)   1.021, assuming all pairs of Cys residues form cystines


Ext. coefficient    21430
Abs 0.1% (=1 g/l)   1.003, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.95
This classifies the protein as stable.



Aliphatic index: 97.97

Grand average of hydropathicity (GRAVY): 0.002