ProtParam
User-provided sequence:
10 20 30 40 50 60
MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGRP INLGLWDTAG
70 80 90 100 110 120
QEDYDRLRPL SYPQTDVFLV CFALNNPASF ENVRAKWYPE VSHHCPNTPI ILVGTKADLR
130 140 150 160 170 180
EDRDTVERLR ERRLQPVSQT QGYVMAKEIK AVKYLECSAL TQRGLKQVFD EAIRAVLTPP
190
QRAKKSKCTV L
References and
documentation are available.
Number of amino acids: 191
Molecular weight: 21454.69
Theoretical pI: 8.50
Total number of negatively charged residues (Asp + Glu): 21
Total number of positively charged residues (Arg + Lys): 24
Atomic composition:
Carbon C 954
Hydrogen H 1518
Nitrogen N 264
Oxygen O 280
Sulfur S 9
Formula: C
954H
1518N
264O
280S
9
Total number of atoms: 3025
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 23295
Abs 0.1% (=1 g/l) 1.086, assuming all pairs of Cys residues form cystines
Ext. coefficient 22920
Abs 0.1% (=1 g/l) 1.068, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.71
This classifies the protein as stable.
Aliphatic index: 87.75
Grand average of hydropathicity (GRAVY): -0.245