ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQAKLLFTRL NFRRPSTTLR QFPLTCFLFH SKAFYSDLVT KEPLITPKRI INKTPGLNLS 

        70         80         90        100        110        120 
ISERASNRLA EIYRNSKENL RISVESGGCH GFQYNLTLEP ATKPDIKNDV KDKEFSDDLD 

       130        140        150        160        170        180 
DDDSKDIIYV LPEDKGRVII DSKSLNILNN TTLTYTNELI GSSFKIINGS LKSSCGCGSS 


FDIEN 

References and documentation are available.

Number of amino acids: 185

Molecular weight: 20876.70

Theoretical pI: 7.63

Amino acid composition: 
Ala (A)   5	  2.7%
Arg (R)  10	  5.4%
Asn (N)  14	  7.6%
Asp (D)  14	  7.6%
Cys (C)   4	  2.2%
Gln (Q)   3	  1.6%
Glu (E)  10	  5.4%
Gly (G)   9	  4.9%
His (H)   2	  1.1%
Ile (I)  16	  8.6%
Leu (L)  21	 11.4%
Lys (K)  15	  8.1%
Met (M)   1	  0.5%
Phe (F)  10	  5.4%
Pro (P)   8	  4.3%
Ser (S)  20	 10.8%
Thr (T)  13	  7.0%
Trp (W)   0	  0.0%
Tyr (Y)   5	  2.7%
Val (V)   5	  2.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 24
Total number of positively charged residues (Arg + Lys): 25

Atomic composition:

Carbon      C	       923
Hydrogen    H	      1479
Nitrogen    N	       251
Oxygen      O	       289
Sulfur      S	         5

Formula: C923H1479N251O289S5
Total number of atoms: 2947

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     7700
Abs 0.1% (=1 g/l)   0.369, assuming all pairs of Cys residues form cystines


Ext. coefficient     7450
Abs 0.1% (=1 g/l)   0.357, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.38
This classifies the protein as stable.



Aliphatic index: 88.54

Grand average of hydropathicity (GRAVY): -0.431