ProtParam
User-provided sequence:
10 20 30 40 50 60
MQCNTSIEDG LDGGDITFPS YSPEAEAQNR FLPRSEFPSV RQLVISKQKS RRRREENVFV
70 80 90 100 110 120
GKMEDVLVKW RPPASSHRGA IEAINGTHPY QLSTAQFNSR RSDPFGSTKR DESVRSIVES
130 140 150 160 170 180
FTDWWKRNSK MFFRDEEEGV RTEHSASQDD QELQLFEEDL FSFHLSPNDM STRNSEHQVQ
190 200 210 220 230 240
TPVLFPYEAP TRTKQNPAER KVIEIGDGEE LSIYRDVYSN PIPVSYLDTL NLNHSMSVRQ
250 260 270
TQQQQQQRQQ QQYVDSSSAF QCCTDSNWAR IFFCHHD
References and
documentation are available.
Number of amino acids: 277
Molecular weight: 32197.41
Theoretical pI: 5.44
Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 32
Atomic composition:
Carbon C 1397
Hydrogen H 2142
Nitrogen N 412
Oxygen O 450
Sulfur S 9
Formula: C
1397H
2142N
412O
450S
9
Total number of atoms: 4410
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 32680
Abs 0.1% (=1 g/l) 1.015, assuming all pairs of Cys residues form cystines
Ext. coefficient 32430
Abs 0.1% (=1 g/l) 1.007, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 73.27
This classifies the protein as unstable.
Aliphatic index: 55.92
Grand average of hydropathicity (GRAVY): -0.936