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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQCNTSIEDG LDGGDITFPS YSPEAEAQNR FLPRSEFPSV RQLVISKQKS RRRREENVFV

70 80 90 100 110 120
GKMEDVLVKW RPPASSHRGA IEAINGTHPY QLSTAQFNSR RSDPFGSTKR DESVRSIVES

130 140 150 160 170 180
FTDWWKRNSK MFFRDEEEGV RTEHSASQDD QELQLFEEDL FSFHLSPNDM STRNSEHQVQ

190 200 210 220 230 240
TPVLFPYEAP TRTKQNPAER KVIEIGDGEE LSIYRDVYSN PIPVSYLDTL NLNHSMSVRQ

250 260 270
TQQQQQQRQQ QQYVDSSSAF QCCTDSNWAR IFFCHHD


References and documentation are available.

Number of amino acids: 277 Molecular weight: 32197.41 Theoretical pI: 5.44
Amino acid composition: 
Ala (A) 11 4.0% Arg (R) 23 8.3% Asn (N) 13 4.7% Asp (D) 18 6.5% Cys (C) 4 1.4% Gln (Q) 24 8.7% Glu (E) 23 8.3% Gly (G) 10 3.6% His (H) 8 2.9% Ile (I) 11 4.0% Leu (L) 14 5.1% Lys (K) 9 3.2% Met (M) 5 1.8% Phe (F) 16 5.8% Pro (P) 15 5.4% Ser (S) 32 11.6% Thr (T) 14 5.1% Trp (W) 4 1.4% Tyr (Y) 7 2.5% Val (V) 16 5.8% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 41 Total number of positively charged residues (Arg + Lys): 32 Atomic composition: Carbon C 1397 Hydrogen H 2142 Nitrogen N 412 Oxygen O 450 Sulfur S 9 Formula: C1397H2142N412O450S9 Total number of atoms: 4410 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 32680 Abs 0.1% (=1 g/l) 1.015, assuming all pairs of Cys residues form cystines Ext. coefficient 32430 Abs 0.1% (=1 g/l) 1.007, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 73.27 This classifies the protein as unstable. Aliphatic index: 55.92 Grand average of hydropathicity (GRAVY): -0.936