ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQCSSSRPVG GKAAGGAEAG GAGATGQMTV TTMEAQVKAA HHHHLHHPTG GGAQHKVEEE 

        70         80         90        100        110        120 
EPDPVEDEMT VLLANKNFHY DVASDLDDDA FVELKEDTAQ ADDAGAGVGF YNPDELLLDD 

       130        140        150        160        170        180 
QPHPQVTWLD DDEIETLDRV TLDMGNMFFN RVDSQDIIYQ QKKKSIKMVG KYIMGDVLGE 

       190        200        210        220        230        240 
GSYGKVKEAM NSENLCRLAV KILTKRKLRR IPNGEQNVTR EIALLKQLKH RHVVELVDVL 

       250        260        270        280        290        300 
YNEEKQKMYL VMEYCVGGLQ EMIDYQPDKR MPLFQAHGYF KQLVDGLEYL HSCRVIHKDI 

       310        320        330        340        350        360 
KPGNLLLSLD QTLKISDFGV AEQLDLFAPD DTCTTGQGSP AFQPPEIANG HETFAGFKVD 

       370        380        390        400        410        420 
IWSSGVTLYN LATGQYPFEG DNIYRLLENI GRGQWEAPAW LYEMDADFAN LILGMLQADP 

       430        440        450        460        470        480 
SKRLSLQEIR HDTWFRSAPV KTGPPIPIPP LKGDKYRNST VIPYLEAYHY GTQEEDVYFT 

       490        500        510        520        530        540 
EHDVNQELAR QAAAAASEIR AKQSAAALAA CHTYEPPSTS AAAASNSLGN GSREEAPVKK 

       550        560 
KGSALKRRAK KLTSCISVRK LSHCRTS 

References and documentation are available.

Number of amino acids: 567

Molecular weight: 62892.94

Theoretical pI: 5.49

Amino acid composition: 
Ala (A)  53	  9.3%
Arg (R)  24	  4.2%
Asn (N)  20	  3.5%
Asp (D)  40	  7.1%
Cys (C)   8	  1.4%
Gln (Q)  29	  5.1%
Glu (E)  41	  7.2%
Gly (G)  43	  7.6%
His (H)  20	  3.5%
Ile (I)  23	  4.1%
Leu (L)  52	  9.2%
Lys (K)  37	  6.5%
Met (M)  15	  2.6%
Phe (F)  16	  2.8%
Pro (P)  28	  4.9%
Ser (S)  30	  5.3%
Thr (T)  28	  4.9%
Trp (W)   5	  0.9%
Tyr (Y)  21	  3.7%
Val (V)  34	  6.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 81
Total number of positively charged residues (Arg + Lys): 61

Atomic composition:

Carbon      C	      2770
Hydrogen    H	      4338
Nitrogen    N	       770
Oxygen      O	       858
Sulfur      S	        23

Formula: C2770H4338N770O858S23
Total number of atoms: 8759

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    59290
Abs 0.1% (=1 g/l)   0.943, assuming all pairs of Cys residues form cystines


Ext. coefficient    58790
Abs 0.1% (=1 g/l)   0.935, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.41
This classifies the protein as unstable.



Aliphatic index: 78.32

Grand average of hydropathicity (GRAVY): -0.487