ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQDSSSYARR YSQAPSSSNL RTTTFGFNGL GTSRTSLAPQ RTLVNARQSI DPDGLSSRVL 

        70         80         90        100        110        120 
TPTMRPSLAP NTRRSSVRVS NASFISQTPN ITSIKDPRPL SDRRYQQECA TQVVNYLLES 

       130        140        150        160        170        180 
GFSQPLGLNN RFMPSTREFA AIFKHLYNKL DPNFRFGARY EEDVTTCLKA LNYPFLDSIS 

       190        200        210        220        230        240 
RSRLVAIGSP HVWPAILGML HWVVSLIQCT EKAVAMVYTV EQNSLDDHLV DKVLFDYLVR 

       250        260        270        280        290        300 
TYHLYLDESP EESEPEKELK ATFNQQNQDL YNQTEALKST NEELINQIKS AEELDSAIQV 

       310        320        330        340        350        360 
LEERYRTMQR DEVKFQSAMS GMKSKMESRT NLMKQLQVNI EEKESQLQLL KEKRDSLKYQ 

       370        380        390        400        410        420 
VENQDISISE FEKMVSEREQ LDRNLNMIGS KISELRKEVF DTDLLIQASI DSLEKKVQKF 

       430        440        450        460        470        480 
NSLAYRIGIV PIAAIRSANN DFELEINPEG PNYINLDLKN KVRPFINEVR RSITLEFHEE 

       490        500        510        520        530        540 
QNKSLKLQEH VDTVNDLIAE LQDELRGIES RLTSVLSECN MLRETASEEK NAFDAESDKL 

       550        560        570        580        590        600 
ERELQQLKLS SHNSMLQLDQ RIQSINIEAD QIAHACMEYK NNIYKEVAFV LGEIIHFKLH 

       610        620 
VQDSLEDLKM DYQKELDDLS RSEL 

References and documentation are available.

Number of amino acids: 624

Molecular weight: 71859.05

Theoretical pI: 5.27

Amino acid composition: 
Ala (A)  33	  5.3%
Arg (R)  40	  6.4%
Asn (N)  39	  6.2%
Asp (D)  36	  5.8%
Cys (C)   5	  0.8%
Gln (Q)  39	  6.2%
Glu (E)  59	  9.5%
Gly (G)  15	  2.4%
His (H)  11	  1.8%
Ile (I)  36	  5.8%
Leu (L)  71	 11.4%
Lys (K)  36	  5.8%
Met (M)  16	  2.6%
Phe (F)  22	  3.5%
Pro (P)  21	  3.4%
Ser (S)  63	 10.1%
Thr (T)  28	  4.5%
Trp (W)   2	  0.3%
Tyr (Y)  19	  3.0%
Val (V)  33	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 95
Total number of positively charged residues (Arg + Lys): 76

Atomic composition:

Carbon      C	      3140
Hydrogen    H	      5030
Nitrogen    N	       882
Oxygen      O	      1003
Sulfur      S	        21

Formula: C3140H5030N882O1003S21
Total number of atoms: 10076

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    39560
Abs 0.1% (=1 g/l)   0.551, assuming all pairs of Cys residues form cystines


Ext. coefficient    39310
Abs 0.1% (=1 g/l)   0.547, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 58.04
This classifies the protein as unstable.



Aliphatic index: 87.50

Grand average of hydropathicity (GRAVY): -0.582