ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQFAGKKTDQ VTTSNPGFEE EEEEEEELQQ DWSQLASLVS KNAALSLPKR GEKDYEPDGT 

        70         80         90        100        110        120 
NLQDLLLYNA SKAMFDTISD SIRGTTVKSE VRGYYVPHKH QAVLLKPKGS FMQTMGRADS 

       130        140        150        160        170        180 
TGELWLDFHE FVYLAERGTI LPYYRLEAGS NKSSKHETEI LLSMEDLYSL FSSQQEMDQY 

       190        200        210        220        230        240 
FVFAHLKRLG FILKPSNQEA AVKTSFFPLK KQRSNLQAIT WRLLSLFKIQ ELSLFSGFFY 

       250        260        270        280        290        300 
SKWNFFFRKY TTSPQLYQGL NRLVRSVAVP KNKKELLDAQ SDREFQKVKD IPLTFKVWKP 

       310        320        330        340        350        360 
HSNFKKRDPG LPDFQVFVYN KNDDLQHFPT YKELRSMFSS LDYKFEFLSE IEDDDDWETN 

       370        380        390        400        410        420 
SYVEDIPRKE YIHKRSAKSQ TEKSESSMKA SFQKKTAQSS TKKKRKAYPP HIQQNRRLKT 

       430        440        450        460 
GYRSFIIAIM DNGLISFVKM SEADFGSESV WYTPNTQKKV DQRWKKH 

References and documentation are available.

Number of amino acids: 467

Molecular weight: 54648.88

Theoretical pI: 9.12

Amino acid composition: 
Ala (A)  22	  4.7%
Arg (R)  22	  4.7%
Asn (N)  17	  3.6%
Asp (D)  27	  5.8%
Cys (C)   0	  0.0%
Gln (Q)  28	  6.0%
Glu (E)  35	  7.5%
Gly (G)  18	  3.9%
His (H)  10	  2.1%
Ile (I)  16	  3.4%
Leu (L)  43	  9.2%
Lys (K)  49	 10.5%
Met (M)  10	  2.1%
Phe (F)  32	  6.9%
Pro (P)  19	  4.1%
Ser (S)  47	 10.1%
Thr (T)  24	  5.1%
Trp (W)   8	  1.7%
Tyr (Y)  20	  4.3%
Val (V)  20	  4.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 71

Atomic composition:

Carbon      C	      2471
Hydrogen    H	      3797
Nitrogen    N	       655
Oxygen      O	       728
Sulfur      S	        10

Formula: C2471H3797N655O728S10
Total number of atoms: 7661

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    73800
Abs 0.1% (=1 g/l)   1.350

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.19
This classifies the protein as unstable.



Aliphatic index: 66.40

Grand average of hydropathicity (GRAVY): -0.758