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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQFFGGSEIS PSPPVPTASG NNAHMMYVFN RNGVCLLYKE WNRPLHTLNP QQDHKLMFGL

70 80 90 100 110 120
LFSLKSLTAK MDPVNADKGN LGVPQLPGQG CSFHSFRTNT YKLSFMETPS GIKIILVTHP

130 140 150 160
KTGDLRESLK YIYSLYVEYV VKNPIYSPGS PIKSELFNTA LDQYVRSIS


References and documentation are available.

Number of amino acids: 169 Molecular weight: 18964.85 Theoretical pI: 9.21
Amino acid composition: 
Ala (A) 5 3.0% Arg (R) 5 3.0% Asn (N) 11 6.5% Asp (D) 5 3.0% Cys (C) 2 1.2% Gln (Q) 6 3.6% Glu (E) 6 3.6% Gly (G) 12 7.1% His (H) 5 3.0% Ile (I) 8 4.7% Leu (L) 18 10.7% Lys (K) 11 6.5% Met (M) 6 3.6% Phe (F) 9 5.3% Pro (P) 14 8.3% Ser (S) 17 10.1% Thr (T) 9 5.3% Trp (W) 1 0.6% Tyr (Y) 9 5.3% Val (V) 10 5.9% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 11 Total number of positively charged residues (Arg + Lys): 16 Atomic composition: Carbon C 861 Hydrogen H 1329 Nitrogen N 223 Oxygen O 244 Sulfur S 8 Formula: C861H1329N223O244S8 Total number of atoms: 2665 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 19035 Abs 0.1% (=1 g/l) 1.004, assuming all pairs of Cys residues form cystines Ext. coefficient 18910 Abs 0.1% (=1 g/l) 0.997, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 38.31 This classifies the protein as stable. Aliphatic index: 80.12 Grand average of hydropathicity (GRAVY): -0.249