ProtParam
User-provided sequence:
10 20 30 40 50 60
MQFFGGSEIS PSPPVPTASG NNAHMMYVFN RNGVCLLYKE WNRPLHTLNP QQDHKLMFGL
70 80 90 100 110 120
LFSLKSLTAK MDPVNADKGN LGVPQLPGQG CSFHSFRTNT YKLSFMETPS GIKIILVTHP
130 140 150 160
KTGDLRESLK YIYSLYVEYV VKNPIYSPGS PIKSELFNTA LDQYVRSIS
References and
documentation are available.
Number of amino acids: 169
Molecular weight: 18964.85
Theoretical pI: 9.21
Total number of negatively charged residues (Asp + Glu): 11
Total number of positively charged residues (Arg + Lys): 16
Atomic composition:
Carbon C 861
Hydrogen H 1329
Nitrogen N 223
Oxygen O 244
Sulfur S 8
Formula: C
861H
1329N
223O
244S
8
Total number of atoms: 2665
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 19035
Abs 0.1% (=1 g/l) 1.004, assuming all pairs of Cys residues form cystines
Ext. coefficient 18910
Abs 0.1% (=1 g/l) 0.997, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.31
This classifies the protein as stable.
Aliphatic index: 80.12
Grand average of hydropathicity (GRAVY): -0.249