ProtParam
User-provided sequence:
10 20 30 40 50 60
MQFLGRSISK SIRPSLNSTA RKSWVLSSQQ FLSTSSTESS SRTRGGGGGN RAEKSSEEWP
70 80 90 100 110 120
RPMEVPYQPK IANSIDLIGY VHQPVQFDST LDGKFWAGTV ISHEPSSDSK SESDSSSNFW
130 140 150 160 170 180
IPVLFEGDLA HTANSYLKKN DRVHITGQIL GDVIQSGANS DQAHVQLFKS FHGSFSHQVM
190 200 210 220 230 240
VRDLHYIEGS KAMPKVLPTL DQNEGVLKHS ASVQRGREFG TNLWFDLVDK PNEWCDYREM
250 260 270 280 290 300
KQNGSVNPKH PDFKKKDGSQ ALWLNNAPTE ILSELKDVKF DIPKYAKQPK AGEESWKDLV
310 320 330 340 350 360
DNMNKWWDNR VDKRTPKSPD FKHKETGVGL WLSDSPSWVL EKLPPPKSKT SDIYGVQEMF
References and
documentation are available.
Number of amino acids: 360
Molecular weight: 40627.48
Theoretical pI: 7.81
Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 46
Atomic composition:
Carbon C 1807
Hydrogen H 2782
Nitrogen N 502
Oxygen O 552
Sulfur S 8
Formula: C
1807H
2782N
502O
552S
8
Total number of atoms: 5651
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 76430
Abs 0.1% (=1 g/l) 1.881, assuming all pairs of Cys residues form cystines
Ext. coefficient 76430
Abs 0.1% (=1 g/l) 1.881, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.44
This classifies the protein as unstable.
Aliphatic index: 64.94
Grand average of hydropathicity (GRAVY): -0.760