ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQGSTQALAE IVRNTEIFLK ESGDELSQED KALIEQKLNE AKVKCEQLNL KAEQSTKELD 

        70         80         90        100        110        120 
KVVTAALKEE TEKVAAARQL EESKTKIENL LNWLSNVEKD SEGSGRLHTQ PLEQNGTHLH 

       130        140        150        160        170        180 
EGDGTSEAGE EDEVNGNLLE TDAEGHGGMT EGNLNQQYEK VKAQHEKIVA QHQAVIMATQ 

       190        200        210        220        230        240 
SAQALLEKQG HHLSPEEKEK LQANVQELKA HYETVLAECE KKVKLTHSLQ EELEKFDADC 

       250        260        270        280        290        300 
GEFEHWLQQS EQELENLEAG ADDLSGLTDK LARQKSFSED VISHKGDLRY ITISGNRVME 

       310        320        330        340        350        360 
AAKSCSKRDG DRIGKDSLET SATHREVQTK LDQVTDRFRC LYSKCSVLGN NLKDLVDKYQ 

       370        380        390        400        410        420 
HYEDASCGLL SGLQACEAKA SRHLLEPIAL DPKNLQRQLE ETKALQGQIS SQQAAVEKLK 

       430        440        450        460        470        480 
KTAEVLLDAK GSLLPAKNDI QKTLDDIVGR YDDLSKSVNE RNEKLQITLT RSLSVQDGLD 

       490        500        510        520        530        540 
EMLDWMGSVE SSLVKQGQVP LNSTALQDLI SKNIMLDQDI TGRQSSINAM NEKVKTFIET 

       550        560        570        580        590        600 
TDPSTASSLQ AKMKDLSARF SEASQKHKEK LAKMVELKSK VEEFEKLSDK LQTFLETQSQ 

       610        620        630        640        650        660 
ALTEVDMPGK DVPELSQHMQ ESTTKFLEHR KDLEALHSLL KEISSHGLPG DKALVFEKTN 

       670        680        690        700        710        720 
NLSKKFKELE DTIQEKKEAL SSCQEQLSAF HDLAQSLKTW IKETTKQVPI VKPSFGTEDL 

       730        740        750        760        770        780 
EKSLEETKKL QEKWSLKTPE IHKANNSGVS LCNLLSALIS PAKAIAAARS GGVILNGEGT 

       790        800        810        820        830        840 
DTNTQDFLAN KGLTSIKTDM TDISHSYEDL GLLLKDKIVE LNTKLSKLQK AQEESSAMMQ 

       850        860        870        880        890        900 
WLQKMNKTAS RWRQTPTPAD SESVKIQVEQ NKSFEAELKQ NVNKVQELKD KLAELLKENP 

       910        920        930        940        950        960 
EAPEAPSWKQ TMAEMDTKWQ ELNQLTIDRQ QKLEESSNNL SQFQTTEAQL KQWLVEKELM 

       970        980        990       1000       1010       1020 
VSVLGPLSTD PNMLNTQKQQ AQILLQEFDT RKPQYEQLTA AGQGILSRPG EDPSLHGIVK 

      1030       1040       1050       1060       1070       1080 
EQLAAVTQKW DNLTGQLRDR CDWIDQAIVK STQYQSLLRS LSGTLTELDE KLSSSLISGT 

      1090       1100       1110       1120       1130       1140 
LPDAVNQQLE AAQKLKQEIE QQTPKIKEAQ TLCEDLSALV KEEYLRAELS RQLEGVLKSF 

      1150       1160       1170       1180       1190       1200 
KDIEQKTENH VQHLQSACAS SHQFQQMSKD FQAWLDAKKE EQRNSPPISA KLDVLESLLK 

      1210       1220       1230       1240       1250       1260 
AQKDFGKTFT EQSNIYEKTV AEGENLLSKT QGAEKAALQL QLNTIKTDWD RFRKQVKERE 

      1270       1280       1290       1300       1310       1320 
EKLKESLEKA LRYREQVDTL QPWVDKCQHS LESVKLSLDP ADTERSITEL KTLQKEMDHH 

      1330       1340       1350       1360       1370       1380 
FGTLELLNNS ANSLLSVCEV DKEVVTEGNK SLIQKVNVVT EQLQSKKVSL ENMAQKFKEF 

      1390       1400       1410       1420       1430       1440 
QEVSKDAKRQ LQDTKEQLEV YQSLGPQACS NKHLTMLQAQ QKSLQTLKHQ VDSARRLAQD 

      1450       1460       1470       1480       1490       1500 
LVMEATDPKG TSDVLSQAET LAEEHRGLSQ QVDEKCSFLE TKLQGLGHFQ NTIREMFSQF 

      1510       1520       1530       1540       1550       1560 
TEFDDELDSM APVGRDVETL QKQKASMQTF LKKLEALITS NDSANRTCKM MLATEETSPD 

      1570       1580       1590       1600       1610       1620 
LIGVKRDLEA LSKQCNKLLD RAKTREEQVS GATEKLEEFH RKLEEFSTLL QKAEEHEESQ 

      1630       1640       1650       1660       1670       1680 
GPVGTETDAI NQQLDVFKIF QKEEIEPLQA KQQDVNWLGQ GLIQSAAANT CTQGLEHDLD 

      1690       1700       1710       1720       1730       1740 
SINARWKTLN KKVAQRTSQL QEALLHCGRF QDALESLLSW MADTEELVAN QKPPSAEFKV 

      1750       1760       1770       1780       1790       1800 
VKAQIQEQKL LQRLLEDRKS TVEAIKREGE KIAASAEPAD RVKLSRQLSL LDSRWEMLLS 

      1810       1820       1830       1840       1850       1860 
RAEARNRQLE GISVVAQEFH ETLEPLNEWL TAVEKKLANS EPIGTQAPKL EEQIAQHKAL 

      1870       1880       1890       1900       1910       1920 
QEDILLRKQS VDQALLNGLE LLKQTTGDEV LIIQDKLEAI KARYKDITKL SADVAKTLEH 

      1930       1940       1950       1960       1970       1980 
ALQLAGQLQS KHKELCNWLD RVEVELLSYE TQGLKGEAAS QVQERQKELK NEVRNNKALL 

      1990       2000       2010       2020       2030       2040 
DSLNEVSSAL LELVPWRARE GLEKTVAEDN ERYRLVSDTI TQKVEEIDAA ILRSQQFDQA 

      2050       2060       2070       2080       2090       2100 
ADAELSWITE TQKKLMSLGC IRLEQDQTSA QLQAQKAFTM DILRHKDIID ELVTSGHKIM 

      2110       2120       2130       2140       2150       2160 
TTSSEEEKQS MKKKLDKVLK KYDAICQINS ERHLQLERAQ SLVSQFWETY EELWPWLMET 

      2170       2180       2190       2200       2210       2220 
QQIISQLPAP ALEYETLRRQ QEEHRQLREL IAEHKPHIDK MNKTGPQLLE LSPKEGVHIQ 

      2230       2240       2250       2260       2270       2280 
EKYVAADTLY SQIKEDVKKR AVVLDEAISQ STQFHDKIDQ ILESLERIAE RLRQPPSISA 

      2290       2300       2310       2320       2330       2340 
EVEKIKEQIG ENKSVSVDME KLQPLYETLR QRGEEMIARS EGTEKDISAK AVQDKLDQMV 

      2350       2360       2370       2380       2390       2400 
FIWGSIHTLV EDREAKLLDV MELAEKFWCD HMSLVVTTKD TQDFIRDLED PGIDPSVVKQ 

      2410       2420       2430       2440       2450       2460 
QQEAAEAIRE EIDGLQEELD MVITLGSELI AACGEPDKPI VKKSIDELNS AWDSLNKAWK 

      2470       2480       2490       2500       2510       2520 
DRIDRLGEAM QAAVQYQDGL QAIFDWVDIA GNKLATMSPI GTDLETVKQQ IEELKQFKSE 

      2530       2540       2550       2560       2570       2580 
AYQQQIEMER LNHQAELLLK KVTEEVDKHT VQDPLMELKL IWDSLDERIV SRQHKLEGAL 

      2590       2600       2610       2620       2630       2640 
LALGQFQHAL DELLAWLTHT KGLLSEQKPV GGDPKAIEIE LAKHHVLQND VLAHQSTVEA 

      2650       2660       2670       2680       2690       2700 
VNKAGNDLIE SSEGEEASNL QYKLRILNQR WQDILEKTDQ RKQQLDSALR QAKGFHGEIE 

      2710       2720       2730       2740       2750       2760 
DLQQWLTDTE RHLLASKPLG GLPETAREQL NAHMEVCTAF AIKEETYKNL MLRGQQMLAR 

      2770       2780       2790       2800       2810       2820 
CPRSVETNID QDITNLKEKW ESVKSKLNEK KTKLEEALHL AMEFHNSLQD FINWLTQAEQ 

      2830       2840       2850       2860       2870       2880 
TLNVASRPSL ILDTILFQID EHKVFANEVN SHREQIIELD KTGTHLKYFS QKQDVVLIKN 

      2890       2900       2910       2920       2930       2940 
LLISVQSRWE KVVQRLVERG RSLDEARKRA KQFHEAWSKL MEWLEDSEKS LDSELEIAND 

      2950       2960       2970       2980       2990       3000 
PDKIKAQLVQ HKEFQKSLGG KHSVYDTTNR TGRSLKEKTS LADDTLKLEN MLSELRDKWD 

      3010       3020       3030       3040       3050       3060 
TICGKSVERQ NKLEEALLFS GQFTDALQAL IDWLYRVEPQ LAEDQPVHGD IDLVMNLIDN 

      3070       3080       3090       3100       3110       3120 
HKVFQKELGK RTSSVQALKR SARELIEGSR DDSSWVRVQM QELSTRWETV CALSISKQTR 

      3130       3140       3150       3160       3170       3180 
LESALQQAEE FHSVVHTLLE WLAEAEQTLR FHGALPDDED ALRTLIEQHK EFMKKLEEKR 

      3190       3200       3210       3220       3230       3240 
AELNKATGMG DALLTACHPD SITTIKHWIT IIQARFEEVL AWAKQHQQRL AGALAGLIAK 

      3250       3260       3270       3280       3290       3300 
QELLENLLAW LQWAETTLTE KDKEVIPQEI EEVKTLIAEH QTFMEEMTRK QPDVDKVTKT 

      3310       3320       3330       3340       3350       3360 
YKRRAADPPS LQSHIPVLDK GRAGRKRFPA SGLYPSGSQT QIETKNPRAN LLVSKWQQVW 

      3370       3380       3390       3400       3410       3420 
LLALERRRKL NDALDRLEEL REFANFDFDI WRKKYMRWMN HKKSRVMDFF RRIDKDQDGK 

      3430       3440       3450       3460       3470       3480 
ITRQEFIDGI LSSKFPTSRL EMSAVADIFD RDGDGYIDYY EFVAALHPNK DAYKPITDAD 

      3490       3500       3510       3520       3530       3540 
KIEDEVTRQV AKCKCAKRFQ VEQIGDNKYR FGDSQQLRLV RILRSTVMVR VGGGWMALDE 

      3550       3560       3570       3580       3590       3600 
FLVKNDPCRV HHHGSKMLRS ESNSSITATQ PTLAKGRTNM ELREKFILAD GASQGMAAFR 

      3610       3620       3630       3640       3650       3660 
PRGRRSRPSS RGASPNRSTS ASSHACQAAS PQVPAAASTP KGTPIQGSKL RLPGYLSGKG 

      3670       3680       3690       3700       3710       3720 
FHSGEDNALI TTAASRVRTQ FAESRKTPSR PGSRAGSKAG SRASSRRGSD ASDFDISEIQ 

      3730       3740       3750       3760 
SVCSDVETVP QTHRPAPRAG SRPSTAKPSK IPTPQRKSPA SKLDKSSKR 

References and documentation are available.

Number of amino acids: 3769

Molecular weight: 427503.37

Theoretical pI: 5.55

Amino acid composition: 
Ala (A) 290	  7.7%
Arg (R) 179	  4.7%
Asn (N) 120	  3.2%
Asp (D) 228	  6.0%
Cys (C)  36	  1.0%
Gln (Q) 305	  8.1%
Glu (E) 389	 10.3%
Gly (G) 152	  4.0%
His (H)  90	  2.4%
Ile (I) 165	  4.4%
Leu (L) 449	 11.9%
Lys (K) 337	  8.9%
Met (M)  69	  1.8%
Phe (F)  85	  2.3%
Pro (P) 100	  2.7%
Ser (S) 286	  7.6%
Thr (T) 213	  5.7%
Trp (W)  54	  1.4%
Tyr (Y)  39	  1.0%
Val (V) 183	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 617
Total number of positively charged residues (Arg + Lys): 516

Atomic composition:

Carbon      C	     18644
Hydrogen    H	     30232
Nitrogen    N	      5302
Oxygen      O	      5967
Sulfur      S	       105

Formula: C18644H30232N5302O5967S105
Total number of atoms: 60250

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   357360
Abs 0.1% (=1 g/l)   0.836, assuming all pairs of Cys residues form cystines


Ext. coefficient   355110
Abs 0.1% (=1 g/l)   0.831, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.63
This classifies the protein as unstable.



Aliphatic index: 85.31

Grand average of hydropathicity (GRAVY): -0.678