ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQHIKHMRTA VRLARYALDH DETPVACIFV HTPTGQVMAY GMNDTNKSLT GVAHAEFMGI 

        70         80         90        100        110        120 
DQIKAMLGSR GVVDVFKDIT LYVTVEPCIM CASALKQLDI GKVVFGCGNE RFGGNGTVLS 

       130        140        150        160        170        180 
VNHDTCTLVP KNNSAAGYES IPGILRKEAI MLLRYFYVRQ NERAPKPRSK SDRVLDKNTF 

       190        200        210        220        230        240 
PPMEWSKYLN EEAFIETFGD DYRTCFANKV DLSSNSVDWD LIDSHQDNII QELEEQCKMF 

       250 
KFNVHKKSKV 

References and documentation are available.

Number of amino acids: 250

Molecular weight: 28252.38

Theoretical pI: 6.70

Amino acid composition: 
Ala (A)  16	  6.4%
Arg (R)  12	  4.8%
Asn (N)  14	  5.6%
Asp (D)  17	  6.8%
Cys (C)   7	  2.8%
Gln (Q)   8	  3.2%
Glu (E)  14	  5.6%
Gly (G)  15	  6.0%
His (H)   8	  3.2%
Ile (I)  14	  5.6%
Leu (L)  17	  6.8%
Lys (K)  18	  7.2%
Met (M)  10	  4.0%
Phe (F)  12	  4.8%
Pro (P)   9	  3.6%
Ser (S)  14	  5.6%
Thr (T)  14	  5.6%
Trp (W)   2	  0.8%
Tyr (Y)   8	  3.2%
Val (V)  21	  8.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      1247
Hydrogen    H	      1960
Nitrogen    N	       344
Oxygen      O	       371
Sulfur      S	        17

Formula: C1247H1960N344O371S17
Total number of atoms: 3939

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    23295
Abs 0.1% (=1 g/l)   0.825, assuming all pairs of Cys residues form cystines


Ext. coefficient    22920
Abs 0.1% (=1 g/l)   0.811, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 27.22
This classifies the protein as stable.



Aliphatic index: 79.12

Grand average of hydropathicity (GRAVY): -0.297