ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQIEMEEKFE DWRGKEAISG KHGGIKAAFI ACVVETMENM VFLACSTNFM MYFTKSMNYS 

        70         80         90        100        110        120 
TPKAATMVTN FVGTSFLLTI FGGFVADSFL TRFAAFVLFG SIELLGLIML TLQAHITKLQ 

       130        140        150        160        170        180 
PQGGKKPSTP QSTVLFTGLY AIAIGVGGVK GSLPAHGGDQ IGTRNQRLIS GFFNWYFFSV 

       190        200        210        220        230        240 
CLGGFLAVTL MVWIEENIGW SSSFTISTAV LASAIFVFVA GCPMYRFKRP AGSPLTRIVN 

       250        260        270        280        290        300 
VFVSAARNRN RFVTDAEVVT QNHNSTDKSI HHNKFKFLNK AKLNNKISAT QVEETRTFLA 

       310        320        330        340        350        360 
LLPIFGSTII MNCCVAQMGT FSVQQGMVTN RKLSRSFEIP VASLNAIPLL CMLSSLALYE 

       370        380        390        400        410        420 
LFGKRILSNS ERSSSFNLKR IGYGLALTSI SMAVAAIVEV KRKHEAVHNN IKISVFWLEL 

       430        440        450        460        470        480 
QFVMLSLSDM LTVGGMLEFF FRESPASMRS MSTALGWCST AMGFFLSSVL VEVVNGITGW 

       490        500        510 
LRDDLNESRL ELFYLVLCVL NTLNLFNYIF WAKRY 

References and documentation are available.

Number of amino acids: 515

Molecular weight: 57160.89

Theoretical pI: 9.38

Amino acid composition: 
Ala (A)  39	  7.6%
Arg (R)  22	  4.3%
Asn (N)  28	  5.4%
Asp (D)   8	  1.6%
Cys (C)   9	  1.7%
Gln (Q)  13	  2.5%
Glu (E)  24	  4.7%
Gly (G)  37	  7.2%
His (H)   8	  1.6%
Ile (I)  32	  6.2%
Leu (L)  55	 10.7%
Lys (K)  24	  4.7%
Met (M)  22	  4.3%
Phe (F)  43	  8.3%
Pro (P)  12	  2.3%
Ser (S)  47	  9.1%
Thr (T)  34	  6.6%
Trp (W)   8	  1.6%
Tyr (Y)  10	  1.9%
Val (V)  40	  7.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 46

Atomic composition:

Carbon      C	      2605
Hydrogen    H	      4070
Nitrogen    N	       670
Oxygen      O	       712
Sulfur      S	        31

Formula: C2605H4070N670O712S31
Total number of atoms: 8088

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    59400
Abs 0.1% (=1 g/l)   1.039, assuming all pairs of Cys residues form cystines


Ext. coefficient    58900
Abs 0.1% (=1 g/l)   1.030, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.71
This classifies the protein as stable.



Aliphatic index: 95.98

Grand average of hydropathicity (GRAVY): 0.362