ProtParam
User-provided sequence:
10 20 30 40 50 60
MQIKEFRVTL PLTVEEYQVA QLFSVAEASK ENTGGGEGIE VLKNEPFEDF PLLGGKYNSG
70 80 90 100 110 120
QYTYKIYHLQ SKVPAYIRLL APKGSLEIHE EAWNAYPYCR TIITNPKFMK DAFKIIIDTL
130 140 150 160 170 180
HVGDAGDSEN VHELTPDKLK VREVVHIDIA NDPVLPADYK PDEDPTTYQS KKTGRGPLVG
190 200 210 220 230 240
SDWKKHVNPV MTCYKLVTCE FKWFGLQTRV ENFIQKSERR LFTNFHRQVF CSTDRWYGLT
250 260 270
MEDIRAIEDQ TKEELDKARQ VGEVWGMRAD AD
References and
documentation are available.
Number of amino acids: 272
Molecular weight: 31302.53
Theoretical pI: 5.60
Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 35
Atomic composition:
Carbon C 1408
Hydrogen H 2173
Nitrogen N 373
Oxygen O 418
Sulfur S 9
Formula: C
1408H
2173N
373O
418S
9
Total number of atoms: 4381
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 45630
Abs 0.1% (=1 g/l) 1.458, assuming all pairs of Cys residues form cystines
Ext. coefficient 45380
Abs 0.1% (=1 g/l) 1.450, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 29.38
This classifies the protein as stable.
Aliphatic index: 77.02
Grand average of hydropathicity (GRAVY): -0.550