ProtParam
User-provided sequence:
10 20 30 40 50 60
MQIKFLTTLA TVLTSVAAMG DLAFNLGVKN DDGTCKDVST FEGDLDFLKS HSKIIKTYAV
70 80 90 100 110 120
SDCNTLQNLG PAAEAEGFQI QLGIWPNDDA HFEAEKEALQ NYLPKISVST IKIFLVGSEA
130 140 150 160 170 180
LYREDLTASE LASKINDIKD LVKGIKDKNG KSYSSVPVGT VDSWNVLVDG ASKPAIDAAD
190 200 210 220 230 240
VVYSNSFSYW QKNSQANASY SLFDDVMQAL QTLQTAKGST DIEFWVGETG WPTDGSSYGD
250 260 270 280 290 300
SVPSVENAAD QWQKGICALR AWGINVAVYE AFDEAWKPDT SGTSSVEKHW GVWQSDKTLK
YSIDCKFN
References and
documentation are available.
Number of amino acids: 308
Molecular weight: 33655.51
Theoretical pI: 4.56
Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 26
Atomic composition:
Carbon C 1504
Hydrogen H 2302
Nitrogen N 382
Oxygen O 481
Sulfur S 7
Formula: C
1504H
2302N
382O
481S
7
Total number of atoms: 4676
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 70150
Abs 0.1% (=1 g/l) 2.084, assuming all pairs of Cys residues form cystines
Ext. coefficient 69900
Abs 0.1% (=1 g/l) 2.077, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 14.23
This classifies the protein as stable.
Aliphatic index: 80.45
Grand average of hydropathicity (GRAVY): -0.263