ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQKETSIFLP SQVTVKVHPV VIFNILDHYI RRNVGQDRVI GTLLGFNNDG VLEIRNCFPV 

        70         80         90        100        110        120 
VHSETEQIAV EMEYQRKMLD LHLKSSPREP IIGWYATGND INENSVHINN FYRDEMGNST 

       130        140        150        160        170        180 
PIHLTVDTGL TNDTMGIHAY MAHNLSLNPE SSLGSYFSQL PLEILTFEAE NAGLESIAQT 

       190        200        210        220        230        240 
KYDQQSTSLL SELESLQGSL TKLDEMLESI TSYIESVEKG EIQGDPRLGR FLAKTIQALP 

       250        260        270        280 
KANAQVMDKV INNSVKDLLM IVYLSSLTRS QLAVATKISH SLSN 

References and documentation are available.

Number of amino acids: 284

Molecular weight: 31754.05

Theoretical pI: 5.18

Amino acid composition: 
Ala (A)  13	  4.6%
Arg (R)  10	  3.5%
Asn (N)  19	  6.7%
Asp (D)  13	  4.6%
Cys (C)   1	  0.4%
Gln (Q)  14	  4.9%
Glu (E)  21	  7.4%
Gly (G)  15	  5.3%
His (H)   9	  3.2%
Ile (I)  22	  7.7%
Leu (L)  33	 11.6%
Lys (K)  12	  4.2%
Met (M)   9	  3.2%
Phe (F)   8	  2.8%
Pro (P)  10	  3.5%
Ser (S)  28	  9.9%
Thr (T)  18	  6.3%
Trp (W)   1	  0.4%
Tyr (Y)   9	  3.2%
Val (V)  19	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 22

Atomic composition:

Carbon      C	      1401
Hydrogen    H	      2238
Nitrogen    N	       378
Oxygen      O	       441
Sulfur      S	        10

Formula: C1401H2238N378O441S10
Total number of atoms: 4468

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    18910
Abs 0.1% (=1 g/l)   0.596, assuming all pairs of Cys residues form cystines


Ext. coefficient    18910
Abs 0.1% (=1 g/l)   0.596, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.35
This classifies the protein as unstable.



Aliphatic index: 99.51

Grand average of hydropathicity (GRAVY): -0.194