ProtParam
User-provided sequence:
10 20 30 40 50 60
MQKQDDFSFK YWTIKVQRGS ILKSHEMENL QSTLGFPPPE MTFGNNYISI EYKNQPVVGF
70 80 90 100 110 120
FTEDALKMVG TKPEDVMQVS FAKDWAKSRI KPGEEYPVIY PFDWTYYTDY QGTIQRPNAK
130 140 150 160 170 180
FIEIDQVIPV QKILNAGQNL WFNEIILFED ELADNGKSMF DVRARVVQGH LILLARLVVR
190 200 210 220 230 240
LDKVNVRLNE TRIYIDLLND FLLKDCRKKQ ATYDKIIKQI PVGGDKAALL DDNNWLSERL
250
DTVDHKIYKL NF
References and
documentation are available.
Number of amino acids: 252
Molecular weight: 29454.84
Theoretical pI: 6.00
Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 33
Atomic composition:
Carbon C 1344
Hydrogen H 2083
Nitrogen N 347
Oxygen O 383
Sulfur S 7
Formula: C
1344H
2083N
347O
383S
7
Total number of atoms: 4164
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 43890
Abs 0.1% (=1 g/l) 1.490, assuming all pairs of Cys residues form cystines
Ext. coefficient 43890
Abs 0.1% (=1 g/l) 1.490, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 30.23
This classifies the protein as stable.
Aliphatic index: 91.63
Grand average of hydropathicity (GRAVY): -0.393