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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQKQDDFSFK YWTIKVQRGS ILKSHEMENL QSTLGFPPPE MTFGNNYISI EYKNQPVVGF

70 80 90 100 110 120
FTEDALKMVG TKPEDVMQVS FAKDWAKSRI KPGEEYPVIY PFDWTYYTDY QGTIQRPNAK

130 140 150 160 170 180
FIEIDQVIPV QKILNAGQNL WFNEIILFED ELADNGKSMF DVRARVVQGH LILLARLVVR

190 200 210 220 230 240
LDKVNVRLNE TRIYIDLLND FLLKDCRKKQ ATYDKIIKQI PVGGDKAALL DDNNWLSERL

250
DTVDHKIYKL NF


References and documentation are available.

Number of amino acids: 252 Molecular weight: 29454.84 Theoretical pI: 6.00
Amino acid composition: 
Ala (A) 11 4.4% Arg (R) 11 4.4% Asn (N) 15 6.0% Asp (D) 21 8.3% Cys (C) 1 0.4% Gln (Q) 14 5.6% Glu (E) 14 5.6% Gly (G) 12 4.8% His (H) 3 1.2% Ile (I) 20 7.9% Leu (L) 23 9.1% Lys (K) 22 8.7% Met (M) 6 2.4% Phe (F) 14 5.6% Pro (P) 11 4.4% Ser (S) 9 3.6% Thr (T) 11 4.4% Trp (W) 5 2.0% Tyr (Y) 11 4.4% Val (V) 18 7.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 35 Total number of positively charged residues (Arg + Lys): 33 Atomic composition: Carbon C 1344 Hydrogen H 2083 Nitrogen N 347 Oxygen O 383 Sulfur S 7 Formula: C1344H2083N347O383S7 Total number of atoms: 4164 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 43890 Abs 0.1% (=1 g/l) 1.490, assuming all pairs of Cys residues form cystines Ext. coefficient 43890 Abs 0.1% (=1 g/l) 1.490, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 30.23 This classifies the protein as stable. Aliphatic index: 91.63 Grand average of hydropathicity (GRAVY): -0.393