ProtParam
User-provided sequence:
10 20 30 40 50 60
MQKSILLTKP DGTQSNLHSI KTETPTTVEF DSEQMERGHR ERGRSKKKRG ERDSNVSSLS
70 80 90 100 110 120
RSRSRASSRS RVREEEFLKW TVLRQDPSMR LRVVDVDSEE EGEGNDEDDD DGDGDDMDEE
130 140 150 160 170 180
ESDEEQVSDI ENDLEIDEEF HYDLGMKVLP NFCTSINEVL DSSKPWIAKY EISIRGHENE
190 200 210 220 230 240
DVSLEQLDGG YVRAMQLLTK GAGAEAGNQR SFILYTDLSS ESTYALTYLM GAAVNQGDTV
250 260 270 280 290 300
YIVHWEPSKP TDDSQMFANV ARIRKHVMHL FDCVAGVLDD LDVVVLSLTH PYPKHLLNEM
310 320 330 340
IHGLKPVALC CSLSVILSTL QNFVCSVPIL AVRKKLKRAK RKGISE
References and
documentation are available.
Number of amino acids: 346
Molecular weight: 39070.70
Theoretical pI: 4.93
Total number of negatively charged residues (Asp + Glu): 64
Total number of positively charged residues (Arg + Lys): 43
Atomic composition:
Carbon C 1684
Hydrogen H 2693
Nitrogen N 481
Oxygen O 557
Sulfur S 15
Formula: C
1684H
2693N
481O
557S
15
Total number of atoms: 5430
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 28670
Abs 0.1% (=1 g/l) 0.734, assuming all pairs of Cys residues form cystines
Ext. coefficient 28420
Abs 0.1% (=1 g/l) 0.727, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.86
This classifies the protein as unstable.
Aliphatic index: 81.07
Grand average of hydropathicity (GRAVY): -0.603