ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQKTNNYKSF KIFFKNVPFR SYPDCKNGKG SGLKAKLAKK IPIEREKFLG IKCLHGKKII 

        70         80         90        100        110        120 
GQISVNSVIG GMRGLPLLFC ETSSLDKNKG IYYRGKLLKD VCAKLPRVQE GTQEGTPEGC 

       130        140        150        160        170        180 
FFLLTSGSMP TKKEAQEVTN EWLKRGSVPR YCLRMIDSMD KRVHPMAQLC AASACLNPQS 

       190        200        210        220        230        240 
QFVEAYTKGA RRADYWKYSY EDSMNLIAML PTVAAAIYSN VFRDGEGSRE VNYEEDWSGN 

       250        260        270        280        290        300 
FCRMLGLPEK DFVDLMRLYM ILHADHESGN VSAHACHLVG TALSDPFLSF SASMCGLAGP 

       310        320        330        340        350        360 
LHGLANQEVL VWLTKLRKAI GDDPSDEELK KFIDDTLKGG QVIPGYGHAV LRDTDPRFVL 

       370        380        390        400        410        420 
QNEFAMKHCK DDPGVKLVTR LWKIIPEVLK KLNKVANPYP NVDAHSGVLL QHYCLKELKF 

       430        440        450        460        470 
YTVLFGVSRA LGVLSQLIWS RALGAPIERP KSFSSIEICK FINEADKKAG KKNNKKKC 

References and documentation are available.

Number of amino acids: 478

Molecular weight: 53553.31

Theoretical pI: 9.27

Amino acid composition: 
Ala (A)  32	  6.7%
Arg (R)  22	  4.6%
Asn (N)  21	  4.4%
Asp (D)  24	  5.0%
Cys (C)  15	  3.1%
Gln (Q)  13	  2.7%
Glu (E)  26	  5.4%
Gly (G)  37	  7.7%
His (H)  11	  2.3%
Ile (I)  23	  4.8%
Leu (L)  50	 10.5%
Lys (K)  49	 10.3%
Met (M)  13	  2.7%
Phe (F)  21	  4.4%
Pro (P)  23	  4.8%
Ser (S)  32	  6.7%
Thr (T)  15	  3.1%
Trp (W)   6	  1.3%
Tyr (Y)  16	  3.3%
Val (V)  29	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 71

Atomic composition:

Carbon      C	      2400
Hydrogen    H	      3809
Nitrogen    N	       655
Oxygen      O	       676
Sulfur      S	        28

Formula: C2400H3809N655O676S28
Total number of atoms: 7568

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    57715
Abs 0.1% (=1 g/l)   1.078, assuming all pairs of Cys residues form cystines


Ext. coefficient    56840
Abs 0.1% (=1 g/l)   1.061, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 28.54
This classifies the protein as stable.



Aliphatic index: 83.85

Grand average of hydropathicity (GRAVY): -0.291