ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQKYDKMEKI GEGTYGTVFK GRNRDTMEIV ALKRVRLDED DEGVPSSALR EICLLKELKH 

        70         80         90        100        110        120 
KNIVRLIDVL HSDKKLTLVF EHCDQDLKKY FDSLNGEIDM AVCRSFMLQL LRGLAFCHSH 

       130        140        150        160        170        180 
NVLHRDLKPQ NLLINKNGEL KLADFGLARA FGIPVKCYSA EVVTLWYRPP DVLFGAKLYT 

       190        200        210        220        230        240 
TSIDMWSAGC ILAELADAGR PLFPGSDVLD QLMKIFRVLG TPNEDSWPGV SHLSDYVALP 

       250        260        270        280        290 
SFPAITSWSQ LVPRLNSKGR DLLQKLLICR PNQRISAEAA MQHPYFTDSS SSGH 

References and documentation are available.

Number of amino acids: 294

Molecular weight: 33180.38

Theoretical pI: 7.63

Amino acid composition: 
Ala (A)  18	  6.1%
Arg (R)  17	  5.8%
Asn (N)  10	  3.4%
Asp (D)  22	  7.5%
Cys (C)   7	  2.4%
Gln (Q)   9	  3.1%
Glu (E)  14	  4.8%
Gly (G)  18	  6.1%
His (H)   9	  3.1%
Ile (I)  14	  4.8%
Leu (L)  40	 13.6%
Lys (K)  20	  6.8%
Met (M)   8	  2.7%
Phe (F)  12	  4.1%
Pro (P)  14	  4.8%
Ser (S)  22	  7.5%
Thr (T)  10	  3.4%
Trp (W)   4	  1.4%
Tyr (Y)   8	  2.7%
Val (V)  18	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	      1484
Hydrogen    H	      2354
Nitrogen    N	       406
Oxygen      O	       426
Sulfur      S	        15

Formula: C1484H2354N406O426S15
Total number of atoms: 4685

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    34295
Abs 0.1% (=1 g/l)   1.034, assuming all pairs of Cys residues form cystines


Ext. coefficient    33920
Abs 0.1% (=1 g/l)   1.022, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.29
This classifies the protein as stable.



Aliphatic index: 95.51

Grand average of hydropathicity (GRAVY): -0.186