ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQLHISPSLR HVTVVTGKGL REFIKVKVGS RRFSYQMVFY SLLFFTFLLR FVFVLSTVDT 

        70         80         90        100        110        120 
IDGDPSPCSS LACLGKRLKP KLLGRRVDSG NVPEAMYQVL EQPLSEQELK GRSDIPQTLQ 

       130        140        150        160        170        180 
DFMSEVKRSK SDAREFAQKL KEMVTLMEQR TRTAKIQEYL YRHVASSSIP KQLHCLALKL 

       190        200        210        220        230        240 
ANEHSINAAA RLQLPEAELV PMLVDNNYFH FVLASDNILA ASVVAKSLVQ NALRPHKIVL 

       250        260        270        280        290        300 
HIITDRKTYF PMQAWFSLHP LSPAIIEVKA LHHFDWLSKG KVPVLEAMEK DQRVRSQFRG 

       310        320        330        340        350        360 
GSSVIVANNK ENPVVVAAKL QALSPKYNSL MNHIRIHLPE LFPSLNKVVF LDDDIVIQTD 

       370        380        390        400        410        420 
LSPLWDIDMN GKVNGAVETC RGEDKFVMSK KFKSYLNFSN PTIAKNFNPE ECAWAYGMNV 

       430        440        450        460        470        480 
FDLAAWRRTN ISSTYYHWLD ENLKSDLSLW QLGTLPPGLI AFHGHVQTID PFWHMLGLGY 

       490        500        510        520        530 
QETTSYADAE SAAVVHFNGR AKPWLDIAFP HLRPLWAKYL DSSDRFIKSC HIRAS 

References and documentation are available.

Number of amino acids: 535

Molecular weight: 60880.43

Theoretical pI: 9.17

Amino acid composition: 
Ala (A)  40	  7.5%
Arg (R)  28	  5.2%
Asn (N)  23	  4.3%
Asp (D)  27	  5.0%
Cys (C)   6	  1.1%
Gln (Q)  21	  3.9%
Glu (E)  25	  4.7%
Gly (G)  21	  3.9%
His (H)  20	  3.7%
Ile (I)  26	  4.9%
Leu (L)  62	 11.6%
Lys (K)  35	  6.5%
Met (M)  14	  2.6%
Phe (F)  28	  5.2%
Pro (P)  28	  5.2%
Ser (S)  45	  8.4%
Thr (T)  20	  3.7%
Trp (W)  10	  1.9%
Tyr (Y)  15	  2.8%
Val (V)  41	  7.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 63

Atomic composition:

Carbon      C	      2763
Hydrogen    H	      4318
Nitrogen    N	       748
Oxygen      O	       764
Sulfur      S	        20

Formula: C2763H4318N748O764S20
Total number of atoms: 8613

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    77725
Abs 0.1% (=1 g/l)   1.277, assuming all pairs of Cys residues form cystines


Ext. coefficient    77350
Abs 0.1% (=1 g/l)   1.271, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.06
This classifies the protein as unstable.



Aliphatic index: 93.85

Grand average of hydropathicity (GRAVY): -0.145