ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQLSWKDIPT VAPANDLLDI VLNRTQRKTP TVIRPGFKIT RIRAFYMRKV KYTGEGFVEK 

        70         80         90        100        110        120 
FEDILKGFPN INDVHPFHRD LMDTLYEKNH YKISLAAISR AKSLVEQVAR DYVRLLKFGQ 

       130        140        150        160        170        180 
SLFQCKQLKR AALGRMATIV KKLRDPLAYL EQVRQHIGRL PSIDPNTRTL LICGYPNVGK 

       190        200        210        220        230        240 
SSFLRCITKS DVDVQPYAFT TKSLYVGHFD YKYLRFQAID TPGILDRPTE EMNNIEMQSI 

       250        260        270        280        290        300 
YAIAHLRSCV LYFMDLSEQC GFTIEAQVKL FHSIKPLFAN KSVMVVINKT DIIRPEDLDE 

       310        320        330        340        350        360 
ERAQLLESVK EVPGVEIMTS SCQLEENVME VRNKACEKLL ASRIENKLKS QSRINNVLNK 

       370        380        390        400        410        420 
IHVAQPQARD DVKRTPFIPE SVKNLKKYDP EDPNRRKLAR DIEAENGGAG VFNVNLKDKY 

       430        440        450        460        470        480 
LLEDDEWKND IMPEILDGKN VYDFLDPEIA AKLQALEEEE EKLENEGFYN SDDEEEIYDG 

       490        500        510        520        530        540 
FEASEVDDIK EKAAWIRNRQ KTMIAEARNR KSLKNKAIMP RSKLTKSFGK MEEHMSTLGH 

       550        560        570        580        590        600 
DMSALQDKQN RAARKNRYVE RGSDVVFGDQ DALTASTENG VKLRQTDRLL DGVADGSMRS 

       610        620        630        640 
KADRMAKMER RERNRHAKQG ESDRHNAVSL SKHLFSGKRG VGKTDFR 

References and documentation are available.

Number of amino acids: 647

Molecular weight: 74410.10

Theoretical pI: 8.91

Amino acid composition: 
Ala (A)  44	  6.8%
Arg (R)  50	  7.7%
Asn (N)  34	  5.3%
Asp (D)  47	  7.3%
Cys (C)   7	  1.1%
Gln (Q)  25	  3.9%
Glu (E)  51	  7.9%
Gly (G)  30	  4.6%
His (H)  13	  2.0%
Ile (I)  38	  5.9%
Leu (L)  57	  8.8%
Lys (K)  58	  9.0%
Met (M)  19	  2.9%
Phe (F)  25	  3.9%
Pro (P)  24	  3.7%
Ser (S)  37	  5.7%
Thr (T)  26	  4.0%
Trp (W)   3	  0.5%
Tyr (Y)  19	  2.9%
Val (V)  40	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 98
Total number of positively charged residues (Arg + Lys): 108

Atomic composition:

Carbon      C	      3272
Hydrogen    H	      5267
Nitrogen    N	       943
Oxygen      O	       985
Sulfur      S	        26

Formula: C3272H5267N943O985S26
Total number of atoms: 10493

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    45185
Abs 0.1% (=1 g/l)   0.607, assuming all pairs of Cys residues form cystines


Ext. coefficient    44810
Abs 0.1% (=1 g/l)   0.602, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.86
This classifies the protein as unstable.



Aliphatic index: 81.99

Grand average of hydropathicity (GRAVY): -0.633