ProtParam
User-provided sequence:
10 20 30 40 50 60
MQLYSSESES DDSQYWVDWF LGLKGNEFFC EVDEDFIQDR FNLTGLSHEV PHYSQSLDLI
70 80 90 100 110 120
LDVLDPDLPE EVQDEVEASA RHLYGLIHAR YILTAQGLYK MLEKYKKCDF GHCPRVLCNG
130 140 150 160 170 180
QPMLPVGLSD IAHTKSVKLY CPRCEDVYTP KSQRHASIDG AYFGTSFPHM LFQVYPELAV
190 200 210 220 230
PKSQERYIPR IFGFKVHSYS ATFKKQDVYK EKQKKRLQGA EAESKNKLAI T
References and
documentation are available.
Number of amino acids: 231
Molecular weight: 26677.28
Theoretical pI: 5.85
Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 27
Atomic composition:
Carbon C 1204
Hydrogen H 1833
Nitrogen N 313
Oxygen O 354
Sulfur S 10
Formula: C
1204H
1833N
313O
354S
10
Total number of atoms: 3714
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 32235
Abs 0.1% (=1 g/l) 1.208, assuming all pairs of Cys residues form cystines
Ext. coefficient 31860
Abs 0.1% (=1 g/l) 1.194, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.42
This classifies the protein as stable.
Aliphatic index: 76.80
Grand average of hydropathicity (GRAVY): -0.490