ProtParam
User-provided sequence:
10 20 30 40 50 60
MQMNPSVTIE RKRVDCSVLP KGWQRDEVRK SGSSANNNAS SNNNSSATAS SNNNNNKVDV
70 80 90 100 110 120
FYYSPTGKRA EGKPQDIAIP DFQPGKMPHC ALPSPSISLY RCSAMPLPIA SGGGNGATSG
130 140 150 160 170 180
SAANALKRKF ARSQGGNAAG AAGAAPPAAT ASSAATATAA SASPSTANRQ QQQIELSRAL
190 200 210 220 230 240
RTDVSLVPPI RQTASIFKQP VTVIRNHKQD PAKAKNEPKH GTREKPKQLF WEKRLERLRA
250 260 270 280 290 300
CHDSGEELDD ISLPKTIRTV GPNVNEQTVL QSVATALHML NAGVHGQSST KADLTKNAMA
310 320 330
FMNPEQPLMH AVIISEDDIR KQEDRVGVAR RKLQDALKT
References and
documentation are available.
Number of amino acids: 339
Molecular weight: 36274.83
Theoretical pI: 9.89
Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 45
Atomic composition:
Carbon C 1548
Hydrogen H 2534
Nitrogen N 486
Oxygen O 496
Sulfur S 12
Formula: C
1548H
2534N
486O
496S
12
Total number of atoms: 5076
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 15720
Abs 0.1% (=1 g/l) 0.433, assuming all pairs of Cys residues form cystines
Ext. coefficient 15470
Abs 0.1% (=1 g/l) 0.426, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 60.34
This classifies the protein as unstable.
Aliphatic index: 67.49
Grand average of hydropathicity (GRAVY): -0.638