ProtParam
User-provided sequence:
10 20 30 40 50 60
MQNAQIKSSS KGSGIDGTDR NSKDGVEKRP LEDVKQMIDA GTPDVGHKST VETKPNVGWQ
70 80 90 100 110 120
ASHSNLAALH EKEQKYEMEH HHARHKLHRQ VIPDYTSAST AMFSDCMFNA APDKVRSLST
130 140 150 160 170 180
MKSSGLSPKH PFNVVATFKG PFPQHSVESK PLDGGYSAKD HFPSFKMLQA QQHPAHRHYK
190 200 210 220 230 240
DNDKYGLKSP SRSFVKDKKR LVHRFLKSME PSSSGQSKDS SALAPAFDPI LPNVISKPSK
250 260 270 280 290 300
RPTHHSHSSD GSSSTQTDIS LQSLLYHDLE SSPKKHVSPS RPPSVASESS PAVANPIGLS
310 320 330 340 350 360
PKDACNASFS QSSSSSLSSS SSSSSSTSFS QSVAVDPLEP PGNITYSSSN LSLNSDELDY
370 380 390 400 410 420
YQRHIGLQLQ QTEALLKHSL KDEVLKDEND LVKNIANFDK IVKELRDLRS RTIGWKELVE
430 440 450 460 470 480
EDYLMNLKQD FDKENPESFE ARLSDTINTN VAKLQDLEKR MASCKDRLAS RKEVMRKMES
490 500 510 520
LLSLENSLMI SKKNVTFASK YRNEALDIVF LIIIIVICYT FKHLVSHK
References and
documentation are available.
Number of amino acids: 528
Molecular weight: 58750.03
Theoretical pI: 8.88
Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 70
Atomic composition:
Carbon C 2562
Hydrogen H 4080
Nitrogen N 734
Oxygen O 815
Sulfur S 17
Formula: C
2562H
4080N
734O
815S
17
Total number of atoms: 8208
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 29130
Abs 0.1% (=1 g/l) 0.496, assuming all pairs of Cys residues form cystines
Ext. coefficient 28880
Abs 0.1% (=1 g/l) 0.492, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 56.93
This classifies the protein as unstable.
Aliphatic index: 70.74
Grand average of hydropathicity (GRAVY): -0.688