ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQNGKIKPEN LLRRSPTNRK LTTGCEILDG CLRGGISCDS LTEIVAESGC GKTQLCLQLS 

        70         80         90        100        110        120 
LCTQLPISHG GLNGSSLYLH SEFPFPFRRL HQLSHTFHQS NPSIYANYND NPCDHVFVQN 

       130        140        150        160        170        180 
VHSVDHLFDI MPRIDGFVGN SKTRFPLKLI VLDSVAALFR SEFDNTPSDL KKRSSLFFKI 

       190        200        210        220        230        240 
SGKLKQLASK FDLAIVITNQ VTDLVETSDG LSGLRIGNLR YLYSSGRRVV PSLGLAWANC 

       250        260        270        280        290        300 
VNSRFFISRS DGSIVKDRSE KDENCSSSVS RSAKRRLDIV FSPYLPGSSC EFMITREGIC 


AVQA 

References and documentation are available.

Number of amino acids: 304

Molecular weight: 33731.54

Theoretical pI: 8.96

Amino acid composition: 
Ala (A)  11	  3.6%
Arg (R)  21	  6.9%
Asn (N)  16	  5.3%
Asp (D)  17	  5.6%
Cys (C)  11	  3.6%
Gln (Q)  10	  3.3%
Glu (E)  11	  3.6%
Gly (G)  21	  6.9%
His (H)   8	  2.6%
Ile (I)  18	  5.9%
Leu (L)  35	 11.5%
Lys (K)  15	  4.9%
Met (M)   3	  1.0%
Phe (F)  17	  5.6%
Pro (P)  13	  4.3%
Ser (S)  39	 12.8%
Thr (T)  13	  4.3%
Trp (W)   1	  0.3%
Tyr (Y)   6	  2.0%
Val (V)  18	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 28
Total number of positively charged residues (Arg + Lys): 36

Atomic composition:

Carbon      C	      1484
Hydrogen    H	      2367
Nitrogen    N	       425
Oxygen      O	       445
Sulfur      S	        14

Formula: C1484H2367N425O445S14
Total number of atoms: 4735

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    15065
Abs 0.1% (=1 g/l)   0.447, assuming all pairs of Cys residues form cystines


Ext. coefficient    14440
Abs 0.1% (=1 g/l)   0.428, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.52
This classifies the protein as unstable.



Aliphatic index: 88.78

Grand average of hydropathicity (GRAVY): -0.183