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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQNSQDYFYA QNRCQQQQAP STLRTVTMAE FRRVPLPPMA EVPMLSTQNS MGSSASASAS

70 80 90 100
SLEMWEKDLE ERLNSIDHDM NNNKFGSGEL KSMFNQGKVE EMDF


References and documentation are available.

Number of amino acids: 104 Molecular weight: 11834.19 Theoretical pI: 4.72
Amino acid composition: 
Ala (A) 7 6.7% Arg (R) 5 4.8% Asn (N) 8 7.7% Asp (D) 5 4.8% Cys (C) 1 1.0% Gln (Q) 9 8.7% Glu (E) 9 8.7% Gly (G) 4 3.8% His (H) 1 1.0% Ile (I) 1 1.0% Leu (L) 7 6.7% Lys (K) 4 3.8% Met (M) 9 8.7% Phe (F) 5 4.8% Pro (P) 5 4.8% Ser (S) 13 12.5% Thr (T) 4 3.8% Trp (W) 1 1.0% Tyr (Y) 2 1.9% Val (V) 4 3.8% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 14 Total number of positively charged residues (Arg + Lys): 9 Atomic composition: Carbon C 501 Hydrogen H 783 Nitrogen N 143 Oxygen O 169 Sulfur S 10 Formula: C501H783N143O169S10 Total number of atoms: 1606 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 8480 Abs 0.1% (=1 g/l) 0.717, assuming all pairs of Cys residues form cystines Ext. coefficient 8480 Abs 0.1% (=1 g/l) 0.717, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 48.60 This classifies the protein as unstable. Aliphatic index: 47.88 Grand average of hydropathicity (GRAVY): -0.788