ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQPRPLFLNS NGAPYGGVFG SIHHCFVKFY IAKCQNMKLI MELVYGSEIS FTDGFAIKPA 

        70         80         90        100        110        120 
RTFVVNTNAP LHSNGFDVQP SAGVATFPHC SNYGDRIFLW RAIGQKLFIE ERSLLYSITD 

       130        140        150        160        170        180 
GSLCIDFTRT PIIPGTSITI FEEGVLCIVV PTASAIHRFY ARLHSKGHDT FSILARIKED 

       190        200        210        220        230        240 
DDFRRFRTSH SLSTPGRPIR ASVTHHPSRN TISYCTAEGQ LVIVTLGSYT ESSDKHEEFT 

       250        260        270        280        290        300 
MGEVGLLGKL LGGTDKRVSD ACVMKNLRPT SGVTRERKIS VQSDIVFAVT RDGWVQAWNV 

       310        320        330        340        350        360 
ETKKQLPSTI DLNNYFASDG KSLNRDLESA DFEEEKSPME PTETVYSIRA YSFDVDILLI 

       370        380        390        400        410        420 
VGCDLAVSGK SVGMRVHILK VSNDQIQHLQ MFETSMSVDE RLVDLELIQN YFPSKESESL 

       430        440        450        460        470        480 
DETQDGELYN PLTAALFSLS ALFKSSSAKK TYSMKRISFA IQWKKGRVFT DFDWHSVRQF 

       490        500        510        520        530        540 
TSPATTKEEP IGNTDEVEPA IERPYNLSAD SSIETLIDVV FDTDIYPFDI VFRAVQIVSD 

       550        560        570        580        590        600 
NFRGTLGQVR NNNWPELSKL VDTYLTSVEF NRKFQQKTDR SIRLRLNAPQ ESQKTALKDF 

       610        620        630        640        650        660 
WWALLRACEE LDFAARGAIS LAPIQFCGDL RIMAVVHRDR MTFLGDNNTE FLEIISSENI 

       670        680        690        700        710        720 
PKQGNVEEKF RKLPNKHFID LIEEASKFAD RRVFLMNRER ARLTNAKNGV AVVEDDNVEN 

       730        740        750        760        770        780 
AYDYDSDGRF INIKTALEGP IVKLAAAFVE ASTFDNSKPF EDPHPQIFGG SFTQSVVSAN 

       790        800        810        820        830        840 
IRVSVESRVH FALTLQTLLN AITEKKSRAG APSFGDVEAL SCELREIIRV YRELNEQLDI 

       850        860        870        880        890        900 
KIVQNGAKMS IGTWLTSDAE GLSMMKKEGG YGPHGYDEIR ENDFNWFVGV TTEAAIRALL 

       910        920        930        940        950        960 
SSSEILVLPR RLVLQKQYKV LLTILNSYIS ETRALKPVIT FYRGIAYSGT DHPVKALNSF 

       970        980        990       1000       1010       1020 
QSCLDAFSEG NNALRKAVYF LLPKRFDVAQ GKDPVEELTA SEYFLTVVRF LQEHNHAEEV 

      1030       1040       1050       1060       1070       1080 
CSVAVKAIEN LPIENESVQL ISNTLFNHLT NRREWFQSLK LILRTTLRSE TRRASIRELL 

      1090       1100       1110       1120       1130       1140 
SLMLACGEWE AIATMKFGEH EQVVEDFLRD AACRQSPSEK KHYFELLFAF YVARKDFRKG 

      1150       1160       1170       1180       1190       1200 
ACAMYEYARH IESTTCMTPE LLRRKRDCLA VVLNLQSVLG MEPDDDRAIY DDATDTNLVF 

      1210       1220       1230       1240       1250       1260 
PEPSDDEDEL ITESDSSGEN GKLPSGNKDS GSGTGNNSSP DNSNGTSSSG TGVDLVALKA 

      1270       1280       1290       1300       1310       1320 
RQMAYALGSD DMDTDDTEDD MKGGSIPLFK RRKLLVLSEK DIRDELVLCS ARVGLLSSDQ 

      1330       1340       1350       1360       1370       1380 
FKGVPPTDLN ELFSLLVDNQ HFDEAFDIAR QFNLDSHRLF FTMTREAIMI DALRDDLNIE 

      1390       1400       1410       1420       1430       1440 
MAATHQAGWV RLNRRHCAAV ATAEEHWSVV RGLVDAAQAE WPGDSRPLRG STEAFLSFKL 

      1450       1460       1470       1480       1490       1500 
NVPVWLHSVF ENNDANDYLR CLVDYESYSI ALQVLSDIVE QETLQASQSN ARTWLPYGII 

      1510       1520       1530       1540       1550       1560 
DELMIRSADY IRKMSLKSPE DQVVAAEVAN LRKSADQKML IYFRKVADFE QAQKMSSRFF 


DK 

References and documentation are available.

Number of amino acids: 1562

Molecular weight: 176074.22

Theoretical pI: 5.36

Amino acid composition: 
Ala (A) 117	  7.5%
Arg (R) 100	  6.4%
Asn (N)  71	  4.5%
Asp (D) 107	  6.9%
Cys (C)  21	  1.3%
Gln (Q)  52	  3.3%
Glu (E) 113	  7.2%
Gly (G)  79	  5.1%
His (H)  32	  2.0%
Ile (I)  88	  5.6%
Leu (L) 148	  9.5%
Lys (K)  77	  4.9%
Met (M)  31	  2.0%
Phe (F)  86	  5.5%
Pro (P)  56	  3.6%
Ser (S) 135	  8.6%
Thr (T)  87	  5.6%
Trp (W)  17	  1.1%
Tyr (Y)  40	  2.6%
Val (V) 105	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 220
Total number of positively charged residues (Arg + Lys): 177

Atomic composition:

Carbon      C	      7813
Hydrogen    H	     12245
Nitrogen    N	      2143
Oxygen      O	      2388
Sulfur      S	        52

Formula: C7813H12245N2143O2388S52
Total number of atoms: 24641

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   154350
Abs 0.1% (=1 g/l)   0.877, assuming all pairs of Cys residues form cystines


Ext. coefficient   153100
Abs 0.1% (=1 g/l)   0.870, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.90
This classifies the protein as unstable.



Aliphatic index: 85.91

Grand average of hydropathicity (GRAVY): -0.287