ProtParam
User-provided sequence:
10 20 30 40 50 60
MQQATGNELL GILDLDNDID FETAYQMLSS NFDDQMSAHI HENTFSATSP PLLTHELGII
70 80 90 100 110 120
PNVATVQPSH VETIPADNQT HHAPLHTHAH YLNHNPHQPS MGFDQALGLK LSPSSSGLLS
130 140 150 160 170 180
TNESNAIEQF LDNLISQDMM SSNASMNSES HLHIRSPKKQ HRYTELNQRY PETHPHSNTG
190 200 210 220 230 240
ELPTNTADVP TEFTTREGPH QPIGNDHYNP PPFSVPEIRI PDSDIPANIE DDPVKVRKWK
250 260 270 280 290 300
HVQMEKIRRI NTKEAFERLI KSVRTPPKEN GKRIPKHILL TCVMNDIKSI RSANEALQHI
LDDS
References and
documentation are available.
Number of amino acids: 304
Molecular weight: 34234.16
Theoretical pI: 5.78
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 25
Atomic composition:
Carbon C 1490
Hydrogen H 2336
Nitrogen N 434
Oxygen O 474
Sulfur S 10
Formula: C
1490H
2336N
434O
474S
10
Total number of atoms: 4744
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 12950
Abs 0.1% (=1 g/l) 0.378, assuming all pairs of Cys residues form cystines
Ext. coefficient 12950
Abs 0.1% (=1 g/l) 0.378, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 54.13
This classifies the protein as unstable.
Aliphatic index: 75.10
Grand average of hydropathicity (GRAVY): -0.737