ProtParam
User-provided sequence:
10 20 30 40 50 60
MQQDDDFQNF VATLESFKDL KSGISGSRIK KLTTYALDHI DIESKIISLI IDYSRLCPDS
70 80 90 100 110 120
HKLGSLYIID SIGRAYLDET RSNSNSSSNK PGTCAHAINT LGEVIQELLS DAIAKSNQDH
130 140 150 160 170 180
KEKIRMLLDI WDRSGLFQKS YLNAIRSKCF AMDISNNTAN TASQQLSLDP KQRSKQILSN
190 200 210 220 230 240
LKKSPPLNLN ISLPTDLTST DPAKQQAALF QVIAALQKHF KTLPSHTSVG TVAPPQAHTI
250 260 270 280 290 300
TEYGSRRERE RERERYNSRR NRSRSPPAPF SQPSTGRKDR YPSVAQDQYS IGAPNTTFGT
310 320 330 340 350 360
NNHHLYPDEL NVSNNPHYRP KPVSYDSTLP PDHIKVYSRT LFIGGVPLNM KEWDLANVLK
370 380 390 400 410 420
PFAEVQSVIL NNSRKHAFVK VYSRHEAENV LQNFNKDGAL PLRTRWGVGF GPRDCCDYQH
430 440 450 460 470 480
GYSIIPMHRL TDADKKWSVS AQWGGTSGQP LVTGIVFEEP DIIVGEGVSS KAISQKMPTD
490 500 510 520 530 540
SGRNGPRSGK PNKSGSISSI SPVPYGNAPL ASPPPQQYVQ PMMQQPYGYA PNQPLPSQGP
550 560 570
AAAAPPVPQQ QFDPTAQLNS LMNMLNQQQQ QQQQS
References and
documentation are available.
Number of amino acids: 575
Molecular weight: 63858.77
Theoretical pI: 9.24
Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 63
Atomic composition:
Carbon C 2805
Hydrogen H 4421
Nitrogen N 811
Oxygen O 867
Sulfur S 15
Formula: C
2805H
4421N
811O
867S
15
Total number of atoms: 8919
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 57550
Abs 0.1% (=1 g/l) 0.901, assuming all pairs of Cys residues form cystines
Ext. coefficient 57300
Abs 0.1% (=1 g/l) 0.897, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 53.70
This classifies the protein as unstable.
Aliphatic index: 72.63
Grand average of hydropathicity (GRAVY): -0.663