ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQRFLLPLLF LALSPPAICH YSSSTSSPSS SSVSSDASEW RPARATYYAA TNPRDAVGGA 

        70         80         90        100        110        120 
CGYGDLVKSG YGMATVGLSE TLFERGQICG ACFELRCVDD LRWCIPGTSI ILTATNFCAP 

       130        140        150        160        170        180 
NYGFDPDGGG HCNPPNKHFV LPIEAFEKIA IWKAGNMPVQ YRRINCRKEG SMRFTVDGGG 

       190        200        210        220        230        240 
IFISVLITNV AGSGDIAAVK IKGSRTGWLP MGRNWGQNWH INADLRNQAL SFEVTSSDRS 

       250        260 
TVTSYNVSPK NWNYGQTFEG KQFETP 

References and documentation are available.

Number of amino acids: 266

Molecular weight: 28975.74

Theoretical pI: 8.35

Amino acid composition: 
Ala (A)  21	  7.9%
Arg (R)  15	  5.6%
Asn (N)  15	  5.6%
Asp (D)  11	  4.1%
Cys (C)   9	  3.4%
Gln (Q)   7	  2.6%
Glu (E)  10	  3.8%
Gly (G)  27	 10.2%
His (H)   4	  1.5%
Ile (I)  15	  5.6%
Leu (L)  17	  6.4%
Lys (K)   9	  3.4%
Met (M)   5	  1.9%
Phe (F)  13	  4.9%
Pro (P)  16	  6.0%
Ser (S)  26	  9.8%
Thr (T)  16	  6.0%
Trp (W)   7	  2.6%
Tyr (Y)   9	  3.4%
Val (V)  14	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 21
Total number of positively charged residues (Arg + Lys): 24

Atomic composition:

Carbon      C	      1285
Hydrogen    H	      1965
Nitrogen    N	       357
Oxygen      O	       382
Sulfur      S	        14

Formula: C1285H1965N357O382S14
Total number of atoms: 4003

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    52410
Abs 0.1% (=1 g/l)   1.809, assuming all pairs of Cys residues form cystines


Ext. coefficient    51910
Abs 0.1% (=1 g/l)   1.791, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.53
This classifies the protein as unstable.



Aliphatic index: 70.08

Grand average of hydropathicity (GRAVY): -0.208