ProtParam
User-provided sequence:
10 20 30 40 50 60
MQRNNRLRNL FTVPVIMARQ LKRNALSAGL AFAGNATSNE FDEHLQNEVE REREIQKKKK
70 80 90 100 110 120
IKRTQSKKSP DLINKSTFQS RTIGSKKEKH RQLDPEYEIV IDGPLRKIKP YHFTYRTFCK
130 140 150 160 170 180
ERWRDKKLVD VFISEFRDRE SEYYKRTIEN GDVHINDETA DLSTVIRNGD LITHQVHRHE
190 200 210 220 230 240
PPVTSRPIKV IFEDDNIMVI DKPSGIPVHP TGRYRFNTIT KMLQNNLGFV VNPCNRLDRL
250 260 270 280 290 300
TSGLMFLAKT PKGADNIGDQ LKAREVTKEY VAKVVGEFPE TEVIVEKPLK LIEPRLALNA
310 320 330 340 350 360
VCQMDEKGAK HAKTVFNRIS YDGKTSIVKC KPLTGRSHQI RVHLQYLGHP IANDPIYSND
370 380 390 400 410 420
EVWGNNLGKG GQADFDIVIT KLDEIGKRKP AKSWFHSNGG YGEVLRQEKC SICESDLYTD
430 440 450 460
PGPNDLDLWL HAYLYESTET EEGTEKKKWC YKTEYPEWAL RR
References and
documentation are available.
Number of amino acids: 462
Molecular weight: 53398.86
Theoretical pI: 9.12
Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 77
Atomic composition:
Carbon C 2370
Hydrogen H 3762
Nitrogen N 680
Oxygen O 700
Sulfur S 13
Formula: C
2370H
3762N
680O
700S
13
Total number of atoms: 7525
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 57215
Abs 0.1% (=1 g/l) 1.071, assuming all pairs of Cys residues form cystines
Ext. coefficient 56840
Abs 0.1% (=1 g/l) 1.064, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.11
This classifies the protein as stable.
Aliphatic index: 77.62
Grand average of hydropathicity (GRAVY): -0.751