ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQRRFLSSIS ATAGNTKTLN QGRWSVKQVK KSNFHVTLDE IRTSIDSSDF IALSLQNTGS 

        70         80         90        100        110        120 
YAAAWHRVSA IDTPQTSYLK AKYAAERYQI LQFALCPFSL QGSKLTVHPY NFHLFPRDEL 

       130        140        150        160        170        180 
KCGMPSYSFS CQASRLTAMA REGFDFNICI YEGISYLSRA QESASKFLSE NPILADSVTV 

       190        200        210        220        230        240 
SSSPATVADT VFVGRIRSRV KNWRQSCIDS GSKTGDDDLV SSLRRLVLGS EQYGSRLCLT 

       250        260        270        280        290        300 
IDVCSERQVQ LILEMLTEFS DDVVPLLVAS KSRGTQAVRT VFMSSKEDKD LFKRELKDLE 

       310        320        330        340        350        360 
KEENRRVRGF REVVDFISSS QKPVVSQNYL SDFTSIHAKF LGPLPSNVDD FSSSLSSAFP 

       370        380        390        400        410        420 
NVVDLSQFMK EISPLSNISN LPAAMSSLNR FFAPVDVEVA NQGCPVKLDE GHQSHGQNAV 

       430        440        450        460        470        480 
MISQLFAKLC TIQKSDLSTI QSNEDFQALA SDEHANSVTS CSKNAGDENV KVWSKNSRRV 

       490        500        510        520        530        540 
SSENLVFIWG LGKKMTAAKL KNVLQKSHPV FAREFDVKYI DRSSAILVFW ESGPSETFLS 

       550        560        570        580        590        600 
AVNNEEQLDG SLREMVAEGL RGAGYETYKR ACRLGFWEAD LAESLDKALE SSDTDPDSDT 

       610 
KPSEIDWSNE LAINFDEL 

References and documentation are available.

Number of amino acids: 618

Molecular weight: 68807.39

Theoretical pI: 5.68

Amino acid composition: 
Ala (A)  45	  7.3%
Arg (R)  34	  5.5%
Asn (N)  28	  4.5%
Asp (D)  40	  6.5%
Cys (C)  11	  1.8%
Gln (Q)  27	  4.4%
Glu (E)  38	  6.1%
Gly (G)  27	  4.4%
His (H)   9	  1.5%
Ile (I)  26	  4.2%
Leu (L)  58	  9.4%
Lys (K)  35	  5.7%
Met (M)  10	  1.6%
Phe (F)  33	  5.3%
Pro (P)  20	  3.2%
Ser (S)  85	 13.8%
Thr (T)  27	  4.4%
Trp (W)   8	  1.3%
Tyr (Y)  13	  2.1%
Val (V)  44	  7.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 78
Total number of positively charged residues (Arg + Lys): 69

Atomic composition:

Carbon      C	      3026
Hydrogen    H	      4762
Nitrogen    N	       836
Oxygen      O	       955
Sulfur      S	        21

Formula: C3026H4762N836O955S21
Total number of atoms: 9600

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    63995
Abs 0.1% (=1 g/l)   0.930, assuming all pairs of Cys residues form cystines


Ext. coefficient    63370
Abs 0.1% (=1 g/l)   0.921, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.42
This classifies the protein as unstable.



Aliphatic index: 80.94

Grand average of hydropathicity (GRAVY): -0.316