ProtParam
User-provided sequence:
10 20 30 40 50 60
MQRSIFARFG NSSAAVSTLN RLSTTAAPHA KNGYATATGA GAAAATATAS STHAAAAAAA
70 80 90 100 110 120
AANHSTQESG FDYEGLIDSE LQKKRLDKSY RYFNNINRLA KEFPLAHRQR EADKVTVWCS
130 140 150 160 170 180
NDYLALSKHP EVLDAMHKTI DKYGCGAGGT RNIAGHNIPT LNLEAELATL HKKEGALVFS
190 200 210 220 230 240
SCYVANDAVL SLLGQKMKDL VIFSDELNHA SMIVGIKHAN VKKHIFKHND LNELEQLLQS
250 260 270 280 290 300
YPKSVPKLIA FESVYSMAGS VADIEKICDL ADKYGALTFL DEVHAVGLYG PHGAGVAEHC
310 320 330 340 350 360
DFESHRASGI ATPKTNDKGG AKTVMDRVDM ITGTLGKSFG SVGGYVAASR KLIDWFRSFA
370 380 390 400 410 420
PGFIFTTTLP PSVMAGATAA IRYQRCHIDL RTSQQKHTMY VKKAFHELGI PVIPNPSHIV
430 440 450 460 470 480
PVLIGNADLA KQASDILINK HQIYVQAINF PTVARGTERL RITPTPGHTN DLSDILINAV
490 500 510 520 530 540
DDVFNELQLP RVRDWESQGG LLGVGESGFV EESNLWTSSQ LSLTNDDLNP NVRDPIVKQL
EVSSGIKQ
References and
documentation are available.
Number of amino acids: 548
Molecular weight: 59362.27
Theoretical pI: 7.09
Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 55
Atomic composition:
Carbon C 2628
Hydrogen H 4153
Nitrogen N 741
Oxygen O 797
Sulfur S 15
Formula: C
2628H
4153N
741O
797S
15
Total number of atoms: 8334
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 44725
Abs 0.1% (=1 g/l) 0.753, assuming all pairs of Cys residues form cystines
Ext. coefficient 44350
Abs 0.1% (=1 g/l) 0.747, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.41
This classifies the protein as stable.
Aliphatic index: 87.66
Grand average of hydropathicity (GRAVY): -0.193