ProtParam
User-provided sequence:
10 20 30 40 50 60
MQSIRSSTRL LQRHRFSISN TLRFTSSSSS SGSWSENESR MVGFDSFPCT RAATYLISSL
70 80 90 100 110 120
KNVGFSSSAI PKQDFKYSLR CFSTTGDSVQ STPQGSPESD CVVPQRIKFK RLDKTAKHIM
130 140 150 160 170 180
QIVDKEAVEE VRNLREIPEI KPGYIVQLKV EVPENKRRVS IVKGVVIARR NAGLNSTFRI
190 200 210 220
RRLVAGVGVE SMFPLYSPNL REIKVVDKKK VRRAKLYYLR DKVNALKKH
References and
documentation are available.
Number of amino acids: 229
Molecular weight: 26030.10
Theoretical pI: 10.40
Total number of negatively charged residues (Asp + Glu): 20
Total number of positively charged residues (Arg + Lys): 43
Atomic composition:
Carbon C 1148
Hydrogen H 1884
Nitrogen N 342
Oxygen O 333
Sulfur S 7
Formula: C
1148H
1884N
342O
333S
7
Total number of atoms: 3714
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 14565
Abs 0.1% (=1 g/l) 0.560, assuming all pairs of Cys residues form cystines
Ext. coefficient 14440
Abs 0.1% (=1 g/l) 0.555, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 58.30
This classifies the protein as unstable.
Aliphatic index: 83.32
Grand average of hydropathicity (GRAVY): -0.450