ProtParam
User-provided sequence:
10 20 30 40 50 60
MQSTATYGST ISSGTSRTLK ITEIGGSNLT SGMSPFGGHA ASAIRESRTR EKKEMSELND
70 80 90 100 110 120
RLASYIEKVR FLEAQNRKME KDLNLLRGKW GHDSTSVKVM YETELRSAKD LIADSDKERA
130 140 150 160 170 180
QLEDQIKKLV EELNNYRNKL HEAERGADVT RKELDNVLGR LGALEAEIEL LKRRISLMEE
190 200 210 220 230 240
SIGHLKRENH RMIDSLHHAR IFQAVEQETL NRIDYQNQVQ TLLEECDFIR RIHDSEIHDL
250 260 270 280 290 300
LAMASRDTTV ENREYFKNEL SSAIREIRSE YDHVSNTTRT DIESWYKLKV QEIHTQNSRN
310 320 330 340 350 360
CLEQNYAREE VKRLRTTLGD MRGKMADLEG RNLLLEKQIE DLNYQMEEDM RSYEQSLNDK
370 380 390 400 410 420
DTSINKLRDE SKILMVELQM LIDTKQTLDA EIVIYRKMLD GEENRAGLRQ LVEQVVKTTS
430 440 450 460 470 480
IHQTKEIESL RVLKGETTSH SSYSRSAKGN IAIQEAEPSG KYIVLENISR RDENIGDWKL
490 500 510 520 530 540
RRKIAGKREI VFTFPREFTL RAQKSVKIFA RGQGVHSPPD SLVYDLEDSF GTGNDVVTTL
550
YNKEGEDRAS HSQRASHT
References and
documentation are available.
Number of amino acids: 558
Molecular weight: 64404.34
Theoretical pI: 6.13
Total number of negatively charged residues (Asp + Glu): 96
Total number of positively charged residues (Arg + Lys): 88
Atomic composition:
Carbon C 2769
Hydrogen H 4512
Nitrogen N 832
Oxygen O 900
Sulfur S 17
Formula: C
2769H
4512N
832O
900S
17
Total number of atoms: 9030
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 40465
Abs 0.1% (=1 g/l) 0.628, assuming all pairs of Cys residues form cystines
Ext. coefficient 40340
Abs 0.1% (=1 g/l) 0.626, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 53.47
This classifies the protein as unstable.
Aliphatic index: 81.97
Grand average of hydropathicity (GRAVY): -0.810