ProtParam
User-provided sequence:
10 20 30 40 50 60
MQSVNNATSS TPIFGVSQPI STAFPKANDV ALTSSLQSSL EEFKSFESKQ ETLLRIKVLK
70 80 90 100 110 120
NLDGLVKDWV QKTTISRIPK DQMFNAGGKL IPFGSYRLGV HSSGADIDSI VVAPRHITRS
130 140 150 160 170 180
DFFNSFKDIL AKNPEVTELC AVEKAFVPIM TLKYSGVDID ILFARLALKS VPEDLNILDD
190 200 210 220 230 240
SLLKNLDEES VRSLNGCRVA EQLLKLVPHQ KNFCITLRAI KLWAKNHGIY SNSMGFFGGI
250 260 270 280 290 300
TWAILVARIC QLYPNAAPSR LIQKVFFIFS TWNWPAPVLL DYINCDRTDL AQLNQLVWDP
310 320 330 340 350 360
RRNHADRYHL MPIITPAFPQ QNSTHNVSRS SMKVIQDEMK KALIICDKIH EGTLEWRDLL
370 380 390 400 410 420
EEINFFSKYH HFIALKLKAE SVKEELAFGG FFESRIRQLV QILEKNQVIQ VAQINPRKFK
430 440 450 460 470 480
DVKDPKKSIW FIGLEFVANV KNVDLTSDIQ QFKMNIDRQS MSVKEIAAGC QVETDFTYEK
490 500 510 520 530 540
RSNLINIISK KDLSCQRIMK KTEVSVPVTK DSRNLKRHIL DEAPTVQKKH KLDVLEKSTV
550
DSLLSTLKKN TSHA
References and
documentation are available.
Number of amino acids: 554
Molecular weight: 63007.96
Theoretical pI: 9.21
Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 75
Atomic composition:
Carbon C 2836
Hydrogen H 4537
Nitrogen N 769
Oxygen O 814
Sulfur S 18
Formula: C
2836H
4537N
769O
814S
18
Total number of atoms: 8974
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 56420
Abs 0.1% (=1 g/l) 0.895, assuming all pairs of Cys residues form cystines
Ext. coefficient 55920
Abs 0.1% (=1 g/l) 0.888, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.90
This classifies the protein as stable.
Aliphatic index: 97.47
Grand average of hydropathicity (GRAVY): -0.209