ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQSWYSMLCP TYKQRNEDFR KLFSKLPEAE RLIVDYSCAL QREILLQGRL YLSENWICFY 

        70         80         90        100        110        120 
SNIFRWETTI SIQLKEVTCL KKEKTAKLIP NAIQICTESE KHFFTSFGAR DRCFLLIFRL 

       130        140        150        160        170        180 
WQNALLEKTL SPRELWHLVH QCYGSELGLT SEDEDYVCPL QLNGLGSPKE VGDMIALSDI 

       190        200        210        220        230        240 
SPSGAADHSQ EPSPVGSRRG RVTPNLSRAS SDADHGAEED KEEQTDSQLD ASSSQTVTPV 

       250        260        270        280        290        300 
AEPLSSEPTP PDGPTSSLGP LDLLSREELL TDTSNSSSST GEEGDLAALL PDLSGRLLIN 

       310        320        330        340        350        360 
SVFHMGAERL QQMLFSDSPF LQGFLQQRKF TDVTLSPWSS DSKCHQRRVL TYTIPISNQL 

       370        380        390        400        410        420 
GPKSASVVET QTLFRRGPQA GGCVVDSEVL TQGIPYQDYF YTAHRYCILG LARNKARLRV 

       430        440        450        460        470        480 
SSEIRYRKQP WSLVKSLIEK NSWSGIEDYF HHLDRELAKA EKLSLEEGGK DTRGLLSGLR 

       490        500        510        520        530        540 
RRKRPLSWRG HRDGPQHPDP DPCTQTSMHT SGSLSSRFSE PSVDQGPGAG IPSALVLISI 

       550        560        570        580        590        600 
VLIVLIALNA LLFYRLWSLE RTAHTFESWH SLALAKGKFP QTATEWAEIL ALQKHFHSVE 

       610        620        630        640 
VHKWRQILRA SVELLDEMKF SLEKLHQGIT VPDPPLDTQP QPDDSFP 

References and documentation are available.

Number of amino acids: 647

Molecular weight: 72825.42

Theoretical pI: 5.99

Amino acid composition: 
Ala (A)  35	  5.4%
Arg (R)  41	  6.3%
Asn (N)  13	  2.0%
Asp (D)  35	  5.4%
Cys (C)  12	  1.9%
Gln (Q)  34	  5.3%
Glu (E)  46	  7.1%
Gly (G)  37	  5.7%
His (H)  19	  2.9%
Ile (I)  28	  4.3%
Leu (L)  83	 12.8%
Lys (K)  29	  4.5%
Met (M)   7	  1.1%
Phe (F)  24	  3.7%
Pro (P)  40	  6.2%
Ser (S)  73	 11.3%
Thr (T)  36	  5.6%
Trp (W)  13	  2.0%
Tyr (Y)  15	  2.3%
Val (V)  27	  4.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 81
Total number of positively charged residues (Arg + Lys): 70

Atomic composition:

Carbon      C	      3234
Hydrogen    H	      5073
Nitrogen    N	       897
Oxygen      O	       981
Sulfur      S	        19

Formula: C3234H5073N897O981S19
Total number of atoms: 10204

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    94600
Abs 0.1% (=1 g/l)   1.299, assuming all pairs of Cys residues form cystines


Ext. coefficient    93850
Abs 0.1% (=1 g/l)   1.289, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.08
This classifies the protein as unstable.



Aliphatic index: 84.42

Grand average of hydropathicity (GRAVY): -0.420