ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQSWYSMLSP TYKQRNEDFR KLFSKLPEAE RLIVDYSCAL QREILLQGRL YLSENWICFY 

        70         80         90        100        110        120 
SNIFRWETTI SIQLKEVTCL KKEKTAKLIP NAIQICTESE KHFFTSFGAR DRCFLLIFRL 

       130        140        150        160        170        180 
WQNALLEKTL SPRELWHLVH QCYGSELGLT SEDEDYVSPL QLNGLGTPKE VGDVIALSDI 

       190        200        210        220        230        240 
TSSGAADRSQ EPSPVGSRRG HVTPNLSRAS SDADHGAEED KEEQVDSQPD ASSSQTVTPV 

       250        260        270        280        290        300 
AEPPSTEPTQ PDGPTTLGPL DLLPSEELLT DTSNSSSSTG EEADLAALLP DLSGRLLINS 

       310        320        330        340        350        360 
VFHVGAERLQ QMLFSDSPFL QGFLQQCKFT DVTLSPWSGD SKCHQRRVLT YTIPISNPLG 

       370        380        390        400        410        420 
PKSASVVETQ TLFRRGPQAG GCVVDSEVLT QGIPYQDYFY TAHRYCILGL ARNKARLRVS 

       430        440        450        460        470        480 
SEIRYRKQPW SLVKSLIEKN SWSGIEDYFH HLERELAKAE KLSLEEGGKD ARGLLSGLRR 

       490        500        510        520        530        540 
RKRPLSWRAH GDGPQHPDPD PCARAGIHTS GSLSSRFSEP SVDQGPGAGI PSALVLISIV 

       550        560        570        580        590        600 
ICVSLIILIA LNVLLFYRLW SLERTAHTFE SWHSLALAKG KFPQTATEWA EILALQKQFH 

       610        620        630        640        650 
SVEVHKWRQI LRASVELLDE MKFSLEKLHQ GITVSDPPFD TQPRPDDSFS 

References and documentation are available.

Number of amino acids: 650

Molecular weight: 72822.40

Theoretical pI: 5.89

Amino acid composition: 
Ala (A)  39	  6.0%
Arg (R)  40	  6.2%
Asn (N)  13	  2.0%
Asp (D)  34	  5.2%
Cys (C)  12	  1.8%
Gln (Q)  33	  5.1%
Glu (E)  47	  7.2%
Gly (G)  38	  5.8%
His (H)  18	  2.8%
Ile (I)  31	  4.8%
Leu (L)  80	 12.3%
Lys (K)  29	  4.5%
Met (M)   4	  0.6%
Phe (F)  25	  3.8%
Pro (P)  40	  6.2%
Ser (S)  72	 11.1%
Thr (T)  36	  5.5%
Trp (W)  13	  2.0%
Tyr (Y)  15	  2.3%
Val (V)  31	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 81
Total number of positively charged residues (Arg + Lys): 69

Atomic composition:

Carbon      C	      3243
Hydrogen    H	      5084
Nitrogen    N	       894
Oxygen      O	       982
Sulfur      S	        16

Formula: C3243H5084N894O982S16
Total number of atoms: 10219

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    94600
Abs 0.1% (=1 g/l)   1.299, assuming all pairs of Cys residues form cystines


Ext. coefficient    93850
Abs 0.1% (=1 g/l)   1.289, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.73
This classifies the protein as unstable.



Aliphatic index: 86.43

Grand average of hydropathicity (GRAVY): -0.364