ProtParam
User-provided sequence:
10 20 30 40 50 60
MQTFDVVVIG GGPGGMTAGM MLKQAGKSVA IIQENHDSFG GVCLNRGCMP TKSMLKAAKV
70 80 90 100 110 120
YRDAQNSEKY GLDLSVNPVD LTRLRAVADA DLNMLRHMVQ GKLTDARIAV FRGKGSFLSE
130 140 150 160 170 180
HELQICQADG SSEQIRGEKI IIATGSVPAE LPCAPFDGHS ILSSDQILKN TDLPHKLLII
190 200 210 220 230 240
GGGAIGCEFA TLYNTFGSRV TLVEAMDSLL PREDKEAGKT LQSTFEQQGI TVKTGAAIKS
250 260 270 280 290 300
ISVEAGTVHV HYDGSCATEE FDKVLVGIGR TANIAGLNLD AAGVATEQGA VKVNEMMQTT
310 320 330 340 350 360
VPHIYALGDV IGGMTLAHAA EKEGYLLAQN LIQGTRHPLD HRAVPRVVFC HPEVAAVGTH
370 380 390 400 410 420
EARAGIKAFT MPQAPNGRAV VDKVAPAFVK LFIEEDTSQI AGAIIIGEGA TEMIHEMAVA
430 440 450
VENRLTLEQI GKTVHAHPTH SKNVLLAVQH FN
References and
documentation are available.
Number of amino acids: 452
Molecular weight: 48080.16
Theoretical pI: 6.05
Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:
Carbon C 2107
Hydrogen H 3407
Nitrogen N 597
Oxygen O 642
Sulfur S 22
Formula: C
2107H
3407N
597O
642S
22
Total number of atoms: 6775
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 9315
Abs 0.1% (=1 g/l) 0.194, assuming all pairs of Cys residues form cystines
Ext. coefficient 8940
Abs 0.1% (=1 g/l) 0.186, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.18
This classifies the protein as stable.
Aliphatic index: 94.12
Grand average of hydropathicity (GRAVY): 0.022