ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQTIKCVVVG DGAVGKTCLL ISYTTNKFPS EYVPTVFDNY AVTVMIGGEP YTLGLFDTAG 

        70         80         90        100        110        120 
QEDYDRLRPL SYPQTDVFLV CFSVVAPASF ENVREKWVPE ISHHCSKTPF LLVGTQVDLR 

       130        140        150        160        170        180 
DDPGMLEKLA KNKQKPVSTD VGEKLAKELK AVKYVECSAL TQKGLKNVFD EAILAALDPP 

       190 
QQEKKKKCNI L 

References and documentation are available.

Number of amino acids: 191

Molecular weight: 21165.50

Theoretical pI: 6.15

Amino acid composition: 
Ala (A)  12	  6.3%
Arg (R)   4	  2.1%
Asn (N)   6	  3.1%
Asp (D)  12	  6.3%
Cys (C)   6	  3.1%
Gln (Q)   8	  4.2%
Glu (E)  12	  6.3%
Gly (G)  11	  5.8%
His (H)   2	  1.0%
Ile (I)   6	  3.1%
Leu (L)  19	  9.9%
Lys (K)  19	  9.9%
Met (M)   3	  1.6%
Phe (F)   8	  4.2%
Pro (P)  12	  6.3%
Ser (S)   9	  4.7%
Thr (T)  13	  6.8%
Trp (W)   1	  0.5%
Tyr (Y)   7	  3.7%
Val (V)  21	 11.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 24
Total number of positively charged residues (Arg + Lys): 23

Atomic composition:

Carbon      C	       953
Hydrogen    H	      1515
Nitrogen    N	       241
Oxygen      O	       283
Sulfur      S	         9

Formula: C953H1515N241O283S9
Total number of atoms: 3001

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    16305
Abs 0.1% (=1 g/l)   0.770, assuming all pairs of Cys residues form cystines


Ext. coefficient    15930
Abs 0.1% (=1 g/l)   0.753, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.47
This classifies the protein as stable.



Aliphatic index: 89.21

Grand average of hydropathicity (GRAVY): -0.153