ProtParam
User-provided sequence:
10 20 30 40 50 60
MQTISGVLPT VLSPSELRSD DERTFQFDEE AEITTHLTES EDLRRLINET AQLGVRVDHI
70 80 90 100 110 120
HDKTDQEIAR LEKVIKEVTE SDTFFRSCSG WFKTNKNFSD SESSSNTQLK SLSQLHGRYD
130 140 150 160 170 180
RDWRQRLNKW FRKNKSKLAL PSDNNLEEVN DDKVYGYGED LMERGKTPYF SDIDDFMNGL
190 200 210 220 230 240
NIISPLTPDD FENDDTLVKI DETCQIHSAS EPEKTSISPT FGKNIKKELV TDDTESIISG
250 260 270 280 290 300
PPLQENKKTL LKYRYVRTSL DMLGSEKSSS KNNSGGMFRI FHKSANFGDK NQENVPRVWD
310 320 330 340 350 360
TLRNNLGREI YLLQGRFKKW TTKHQNLKKG QPCKDEDAVT VPLPSSDPGK ETQLETKLCF
370
VPEPGDQPLV QA
References and
documentation are available.
Number of amino acids: 372
Molecular weight: 42594.46
Theoretical pI: 5.32
Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 52
Atomic composition:
Carbon C 1861
Hydrogen H 2937
Nitrogen N 521
Oxygen O 606
Sulfur S 9
Formula: C
1861H
2937N
521O
606S
9
Total number of atoms: 5934
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 38180
Abs 0.1% (=1 g/l) 0.896, assuming all pairs of Cys residues form cystines
Ext. coefficient 37930
Abs 0.1% (=1 g/l) 0.890, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.29
This classifies the protein as unstable.
Aliphatic index: 68.87
Grand average of hydropathicity (GRAVY): -0.892