ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQTLMSHSRI TPLPGAITKE EIKNQLLVHE RRFLSKPNRK DQWNVLPQSA HSKNTVNPVR 

        70         80         90        100        110        120 
KIADACAVPP HPEKKVIKLH LGDPSVGGKL PPSEIAVQAM HESVSSHMFD GYGPAVGALA 

       130        140        150        160        170        180 
AREAIVERYS SADNVFTADD VVLASGCSHA LQMAIEAVAN AGENILVPHP GFPLYSTLCR 

       190        200        210        220        230        240 
PHNIVDKPYK IDMTGEDVRI DLSYMATIID DNTKAIIVNN PGNPTGGVFT KEHLEEILAF 

       250        260        270        280        290        300 
AHQYKLIIIA DEIYGDLVYN GATFYPLASL SPKVPIITCD GIAKRWMVPG WRLGWLIIHN 

       310        320        330        340        350        360 
HFGVLTDVKN GIVALSQKIV GPCSLVQGAL PKILRETPED YFVYTRNVIE TNANIVDSIL 

       370        380        390        400        410        420 
ADVPGMRVVK PKGAMYMMVN ISRTAYGSDV SFCQNLIREE SVFCLPGQAF SAPGYFRVVL 

       430        440        450        460 
TCGSEDMEEA ALRIREFCYR NFNQHSDSED SSDEGLDLSA MESD 

References and documentation are available.

Number of amino acids: 464

Molecular weight: 51031.46

Theoretical pI: 5.69

Amino acid composition: 
Ala (A)  39	  8.4%
Arg (R)  20	  4.3%
Asn (N)  23	  5.0%
Asp (D)  27	  5.8%
Cys (C)   9	  1.9%
Gln (Q)  12	  2.6%
Glu (E)  27	  5.8%
Gly (G)  30	  6.5%
His (H)  15	  3.2%
Ile (I)  35	  7.5%
Leu (L)  36	  7.8%
Lys (K)  22	  4.7%
Met (M)  14	  3.0%
Phe (F)  15	  3.2%
Pro (P)  30	  6.5%
Ser (S)  33	  7.1%
Thr (T)  19	  4.1%
Trp (W)   4	  0.9%
Tyr (Y)  15	  3.2%
Val (V)  39	  8.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 54
Total number of positively charged residues (Arg + Lys): 42

Atomic composition:

Carbon      C	      2271
Hydrogen    H	      3575
Nitrogen    N	       615
Oxygen      O	       675
Sulfur      S	        23

Formula: C2271H3575N615O675S23
Total number of atoms: 7159

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    44850
Abs 0.1% (=1 g/l)   0.879, assuming all pairs of Cys residues form cystines


Ext. coefficient    44350
Abs 0.1% (=1 g/l)   0.869, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.17
This classifies the protein as unstable.



Aliphatic index: 92.46

Grand average of hydropathicity (GRAVY): -0.083