ProtParam
User-provided sequence:
10 20 30 40 50 60
MQVLRQLPHQ HQLASSPSSS CDIKSIYASE KVREAIRDHE QTIATSTAPK HKAKVVVLGD
70 80 90 100 110 120
SGVGKTSIIY RHRYGAHYRP VNATIGASFV SFDVGADYRD REDVVRLQVW DTAGQERFRC
130 140 150 160 170 180
MVPMYMRNAD AALIVYDVTD RNTFEDVEKW LKDLDRSSGT EDANVYLIGN KTDLVEKREV
190 200 210 220 230 240
TEAEGKAMAA KINAKFFELS NDQPNLFAAI LSELSDDVLQ SRQESSEKKT DLQLIRKEKV
250 260
SLGDDKFEDN PNIQLKIQKS KCCSML
References and
documentation are available.
Number of amino acids: 266
Molecular weight: 30036.90
Theoretical pI: 6.11
Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 38
Atomic composition:
Carbon C 1308
Hydrogen H 2097
Nitrogen N 375
Oxygen O 415
Sulfur S 10
Formula: C
1308H
2097N
375O
415S
10
Total number of atoms: 4205
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 23170
Abs 0.1% (=1 g/l) 0.771, assuming all pairs of Cys residues form cystines
Ext. coefficient 22920
Abs 0.1% (=1 g/l) 0.763, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.36
This classifies the protein as unstable.
Aliphatic index: 81.02
Grand average of hydropathicity (GRAVY): -0.547