ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MQYKKSLVAS ALVATSLAAY APKDPWSTLT PSATYKGGIT DYSSTFGIAV EPIATTASSK 

        70         80         90        100        110        120 
AKRAAAISQI GDGQIQATTK TTAAAVSQIG DGQIQATTKT KAAAVSQIGD GQIQATTKTT 

       130        140        150        160        170        180 
SAKTTAAAVS QIGDGQIQAT TKTKAAAVSQ IGDGQIQATT KTTAAAVSQI GDGQIQATTK 

       190        200        210        220        230        240 
TTAAAVSQIG DGQIQATTNT TVAPVSQITD GQIQATTLTS ATIIPSPAPA PITNGTDPVT 

       250        260        270        280        290        300 
AETCKSSGTL EMNLKGGILT DGKGRIGSIV ANRQFQFDGP PPQAGAIYAA GWSITPEGNL 

       310        320        330        340 
AIGDQDTFYQ CLSGNFYNLY DEHIGTQCNA VHLQAIDLLN C 

References and documentation are available.

Number of amino acids: 341

Molecular weight: 34621.64

Theoretical pI: 6.10

Amino acid composition: 
Ala (A)  54	 15.8%
Arg (R)   3	  0.9%
Asn (N)   9	  2.6%
Asp (D)  17	  5.0%
Cys (C)   4	  1.2%
Gln (Q)  32	  9.4%
Glu (E)   5	  1.5%
Gly (G)  32	  9.4%
His (H)   2	  0.6%
Ile (I)  31	  9.1%
Leu (L)  14	  4.1%
Lys (K)  18	  5.3%
Met (M)   2	  0.6%
Phe (F)   5	  1.5%
Pro (P)  14	  4.1%
Ser (S)  25	  7.3%
Thr (T)  50	 14.7%
Trp (W)   2	  0.6%
Tyr (Y)   8	  2.3%
Val (V)  14	  4.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 22
Total number of positively charged residues (Arg + Lys): 21

Atomic composition:

Carbon      C	      1499
Hydrogen    H	      2433
Nitrogen    N	       415
Oxygen      O	       510
Sulfur      S	         6

Formula: C1499H2433N415O510S6
Total number of atoms: 4863

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    23170
Abs 0.1% (=1 g/l)   0.669, assuming all pairs of Cys residues form cystines


Ext. coefficient    22920
Abs 0.1% (=1 g/l)   0.662, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 29.06
This classifies the protein as stable.



Aliphatic index: 79.21

Grand average of hydropathicity (GRAVY): -0.107