ProtParam
User-provided sequence:
10 20 30 40 50 60
MQYKKTLVAS ALAATTLAAY APSEPWSTLT PTATYSGGVT DYASTFGIAV QPISTTSSAS
70 80 90 100 110 120
SAATTASSKA KRAASQIGDG QVQAATTTAS VSTKSTAAAV SQIGDGQIQA TTKTTAAAVS
130 140 150 160 170 180
RDGQIQATTK TTSAKTTAAA VSQISDGQIQ ATTTTLAPKS TAAAVSQIGD GQVQATTKTT
190 200 210 220 230 240
AAAVSQIGDG QVQATTKTTA AAVSQIGDGQ VQATTKTTAA AVSQIGDGQV QATTKTTAAA
250 260 270 280 290 300
VSQITDGQVQ ATTKTTQAAS QVSDGQVQAT TATSASAAAT STDPVDAVSC KTSGTLEMNL
310 320 330 340 350 360
KGGILTDGKG RIGSIVANRQ FQFDGPPPQA GAIYAAGWSI TPDGNLAIGD NDVFYQCLSG
370 380
TFYNLYDEHI GSQCTPVHLE AIDLIDC
References and
documentation are available.
Number of amino acids: 387
Molecular weight: 38618.50
Theoretical pI: 5.14
Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 21
Atomic composition:
Carbon C 1646
Hydrogen H 2680
Nitrogen N 464
Oxygen O 591
Sulfur S 6
Formula: C
1646H
2680N
464O
591S
6
Total number of atoms: 5387
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 23170
Abs 0.1% (=1 g/l) 0.600, assuming all pairs of Cys residues form cystines
Ext. coefficient 22920
Abs 0.1% (=1 g/l) 0.593, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 27.21
This classifies the protein as stable.
Aliphatic index: 72.09
Grand average of hydropathicity (GRAVY): -0.094