ProtParam
User-provided sequence:
10 20 30 40 50 60
MQYVGRAFDS VTKTWNAINP STLSGAIDVI VVEQEDKTLA CSPFHVRFGK FSLLLPSDKK
70 80 90 100 110 120
VEFSVNGQLT GFNMKLGDGG EAFFVFATEN AVPRELQTSP IVSPTTSPKQ TPSINVTEPQ
130 140 150 160 170 180
DLELDKVSQD HEKDQSNTYL MEDGYEFPLT RDLIRRSKSD ADQTPPTGFK HLRHSSCLEM
190 200 210 220 230 240
AGSDRTPSMP ATTLADLRLL QKAKELGKRL SGKELPTRVG DNGDVMLDMT GYKSSAANIN
250 260 270 280 290 300
IAELARETFK DEFPMIEKLL REDEEGNLWF HASEDAKKFA EVYGHSPPAS PSRTPASPKS
310 320 330 340 350 360
DSALMDEDSD LSRRHSLSEQ SLSPVSESYP QYAKTLRLTS DQLRSLNLKP GKNELSFGVN
370 380 390 400 410 420
GGKAICTANL FFWKHNDPVV ISDIDGTITK SDALGHMFTL IGKDWTHAGV AKLYTDITNN
430 440 450 460 470 480
GYKIMYLTSR SVGQADSTRH YLRNIEQNGY SLPDGPVILS PDRTMAALHR EVILRKPEVF
490 500 510 520 530 540
KMACLRDLCN IFALPVPRTP FYAGFGNRIT DAISYNHVRV PPTRIFTINS AGEVHIELLQ
550 560 570 580 590 600
RSGHRSSYVY MNELVDHFFP PIEVSTRDEV SSFTDVNFWR SPLLELSDEE EDDTNKSTSK
610 620 630 640 650
SPKTPKNTKF GYQEFEGIDE EDAQDYSPSP LIKSFNELMF EGEEDEEGEE DVENAV
References and
documentation are available.
Number of amino acids: 656
Molecular weight: 73352.05
Theoretical pI: 5.05
Total number of negatively charged residues (Asp + Glu): 100
Total number of positively charged residues (Arg + Lys): 71
Atomic composition:
Carbon C 3235
Hydrogen H 5038
Nitrogen N 878
Oxygen O 1030
Sulfur S 20
Formula: C
3235H
5038N
878O
1030S
20
Total number of atoms: 10201
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 54570
Abs 0.1% (=1 g/l) 0.744, assuming all pairs of Cys residues form cystines
Ext. coefficient 54320
Abs 0.1% (=1 g/l) 0.741, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 59.82
This classifies the protein as unstable.
Aliphatic index: 72.84
Grand average of hydropathicity (GRAVY): -0.548